Product Pathways - Akt Signaling
GSK-3 Fusion Protein #9237
Molecular Weight
27 kDa
Description
GSK-3 Fusion Protein serves as a useful substrate for assaying Akt kinase activity. The GSK-3 α/β crosstide, corresponding to residues surrounding GSK-3 α/β (Ser21/9) (CGPKGPGRRGRRRTSSFAEG) is expressed as a GST fusion protein.
Western Blotting
Akt kinase activity in PDGF-treated NIH/3T3 cell extracts was analyzed by IP/kinase assay. Cell extracts (200 μl) were incubated overnight with Immobilized Akt 1G1 Mouse mAb #9279. After extensive washing the kinase reaction was performed in the presence of 200 μM of cold ATP and 1 μg or 0.5 μg of GSK-substrate. Phosphorylation of GSK-3 was measured by Western blot using Phospho-GSK-3α/β (Ser21/9) Antibody.
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is an ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
- Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
- Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
- Cross, D.A. et al. (1995) Nature 378, 785-789.
- Nusse, R. (1997) Cell 89, 321-323.
- Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.
Application References
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Companion Products
- 9840 Akt Kinase Assay Kit (Nonradioactive)
- 9331 Phospho-GSK-3α/β (Ser21/9) Antibody
- 9336 Phospho-GSK-3β (Ser9) Antibody
- 7535 Akt1 Kinase
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
