Product Pathways - NF-kappaB Signaling

IkappaB-alpha Antibody #9242

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP IHC-P	H M R Mk B Dg (Pg)	Endogenous	39	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig  B=Bovine  Dg=Dog
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

IkappaB-alpha Antibody detects endogenous levels of total IkappaB-alpha protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide (KLH-coupled) derived from the sequence of human IkappaB-alpha. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκB-α at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκB-α phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

Application References

• Bonnard, M. et al. (2000) Deficiency of T2K leads to apoptotic liver degeneration and impaired NF-kappaB-dependent gene transcription. EMBO J. 19, 4976-4985. This article references the use of IkappaB-alpha Antibody in the following applications: Western Blotting
• Burns, K. et al. (2000) Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. Nat. Cell Biol. 2, 346-351. This article references the use of IkappaB-alpha Antibody in the following applications: Western Blotting
• Delehedde, M. et al. (2000) Fibroblast growth factor-2 stimulation of p42/44MAPK phosphorylation and ikappa B degradation is regulated by heparan sulfate/heparin in rat mammary fibroblasts. J. Biol. Chem. 275, 33905-33910. This article references the use of IkappaB-alpha Antibody in the following applications: Western Blotting
• Hoeflich, K. P. et al. (2000) Requirement for glycogen synthase kinase-3beta in cell survival and NF-kappaB activation. Nature 406, 86-90. This article references the use of IkappaB-alpha Antibody in the following applications: Western Blotting
• Todd, D. E. et al. (2004) ERK1/2 and p38 cooperate to induce a p21CIP1-dependent G1 cell cycle arrest. Oncogene 23, 3284-3295. This article references the use of IkappaB-alpha Antibody in the following applications: Western Blotting
• Casciati, A. et al. (2002) Oxidative modulation of nuclear factor-kappaB in human cells expressing mutant fALS-typical superoxide dismutases. J Neurochem 83, 1019-1029. This article references the use of IkappaB-alpha Antibody in the following applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

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• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.