Product Pathways - NF-kB Signaling
NF-κB Control Cell Extracts #9243
Untreated NF-κB Control Cell Extracts:Total cell extracts from HeLa cells prepared without treatment serve as a negative control. Supplied in blue SDS Sample Buffer.TNF-treated NF-κB Control Cell Extracts:Total cell extracts from HeLa cells prepared with Human Tumor Necrosis Factor-α (hTNF-α) #8902 treatment serve as a positive control. Supplied in red SDS Sample Buffer.
Western blot analysis of NF-κB Control Cell Extracts #9243, using IKKα Antibody #2682 (upper), IKKβ (2C8) Rabbit mAb #2370 (middle) and Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 (lower).
Western blot analysis of NF-κB Control Cell Extracts #9243, using IκBα (44D4) Rabbit mAb #4812 (left) and Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (right).
Western blot analysis of NF-κB Control Cell Extracts #9243, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (left) and NF-κB p65 (E498) Antibody #3987 (right).
Western Blots: CST recommends using 20 µl of untreated and TNF-treated NF-κB cell extracts as controls.
Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).
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- Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
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- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Senftleben, U. et al. (2001) Science 293, 1495-9.
- Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
- Xiao, G. et al. (2001) Mol Cell 7, 401-9.
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- 2682 IKKα Antibody
- 2370 IKKβ (2C8) Rabbit mAb
- 2697 Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb
- 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- 3039 Phospho-NF-κB p65 (Ser468) Antibody
- 4954 RelB Antibody
- 4727 c-Rel Antibody
- 3035 NF-κB1 p105/p50 Antibody
- 4806 Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb
- 4882 NF-κB2 p100/p52 Antibody
- 4812 IκBα (44D4) Rabbit mAb
- 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8902 Human Tumor Necrosis Factor-α (hTNF-α)
For Research Use Only. Not For Use In Diagnostic Procedures.