Product Pathways - NF-kB Signaling

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Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W	H M R Mk (B) (Dg) (Pg) (GP)	Endogenous	40	Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Dg=Dog  Pg=Pig  GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

9246:

Western Blotting

Specificity / Sensitivity

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb detects endogenous levels of IκBα only when phosphorylated at Ser32/36.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

Application References

• Howe, C.J. et al. (2002) J Biol Chem 277, 30469-76. Applications: Western Blotting
• Casciati, A. et al. (2002) J Neurochem 83, 1019-29. Applications: Western Blotting
• Gao, H. et al. (2004) Mol Cell 14, 303-17. Applications: Western Blotting
• Nair, A. et al. (2003) Oncogene 22, 50-8. Applications: IHC-P (paraffin)
• Schultz, C. et al. (2006) Mol Cell Neurosci 33, 68-80. Applications: Western Blotting
• Basak, S. et al. (2007) Cell 128, 369-81. Applications: Western Blotting
• Chen, Y. et al. (2007) J Immunol 178, 49-57. Applications: Western Blotting
• Wright, A. et al. (2007) Dev Cell 13, 705-16. Applications: IHC-P (paraffin)
• Lou, H. and Kaplowitz, N. (2007) J Biol Chem 282, 29470-81. Applications: Kinase Assay Western Blotting
• Saitoh, Y. et al. (2008) Blood 111, 5118-29. Applications: Western Blotting
• Luedde, T. et al. (2008) Proc Natl Acad Sci U S A 105, 9733-8. Applications: Western Blotting
• Vince, J.E. et al. (2008) J Cell Biol 182, 171-84. Applications: Western Blotting
• Mabilleau, G. and Sabokbar, A. (2009) PLoS ONE 4, e4173. Applications: Western Blotting
• Tokunaga, F. et al. (2009) Nat Cell Biol 11, 123-32. Applications: Western Blotting
• Chiron, D. et al. (2009) J Immunol 182, 4471-8. Applications: Western Blotting
• Turner, N.A. et al. (2009) Am J Physiol Heart Circ Physiol 297, H1117-27. Applications: Western Blotting
• Kwong, C. et al. (2011) J Immunol 186, 1781-9. Applications: Western Blotting
• Gardner, A. et al. (2012) Am J Pathol 180, 2293-308. Applications: Western Blotting
• Tokunaga, F. et al. (2012) EMBO J 31, 3856-70. Applications: Western Blotting
• Shibata, Y. et al. (2012) Nat Commun 3, 1061. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
• 4812 IκBα (44D4) Rabbit mAb
• 4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)
• 9242 IκBα Antibody
• 9247 IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen)
• 4764 NF-κB p65 (C22B4) Rabbit mAb
• 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
• 9243 NF-κB Control Cell Extracts
• 9936 NF-κB Pathway Sampler Kit
• 1146 IκBα (Ser32) Biotinylated Peptide
• 7072 Phototope^®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
• 7076 Anti-mouse IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8125 SignalStain^® Boost IHC Detection Reagent (HRP, Mouse)
• 8219 PhosphoPlus^® IκBα (Ser32/Ser36) Antibody Duet

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For Research Use Only. Not For Use In Diagnostic Procedures.