Product Pathways - NF-kB Signaling
IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) #9247
PhosphoSitePlus® protein, site, and accession data: IkB-alpha
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 39 | Mouse IgG2a |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) detects endogenous levels of total IκBα protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human IκBα.
Western Blotting
Western blot analysis of extracts from Hela cells, treated with TNF-α (#2169, 10 ng/ml) for the indicated times, using Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (upper) or IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) (lower).
IP
Western blot analysis of extracts from HT29 cells (lane 1), protein A alone immunoprecipitation (lane 2), immunoprecipitation with nonspecific mouse IgG2a (lane 3), or immunoprecipitation with IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) (lane 4). Western blot was performed using IκBα Antibody #9242.
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
- Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
Application References
- Gao, H. et al. (2004) Identification of beta-arrestin2 as a G protein-coupled receptor-stimulated regulator of NF-kappaB pathways. Mol. Cell 14, 303-317. Applications: IP Western Blotting
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Companion Products
- 4812 IκBα (44D4) Rabbit mAb
- 4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)
- 9242 IκBα Antibody
- 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
- 9246 Phospho-IκBα (Ser32/36) (5A5) Mouse mAb
- 4764 NF-κB p65 (C22B4) Rabbit mAb
- 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- 9243 NF-κB Control Cell Extracts
- 9936 NF-κB Pathway Sampler Kit
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
