Product Pathways - NF-kB Signaling
IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) #9247
|9247S||100 µl (10 western blots)||---||In Stock||---|
|9247||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||39||Mouse IgG2a|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Hamster, Monkey.
Specificity / Sensitivity
IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) detects endogenous levels of total IκBα protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human IκBα.
Western blot analysis of extracts from Hela cells, treated with TNF-α (#2169, 10 ng/ml) for the indicated times, using Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (upper) or IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) (lower).
Western blot analysis of extracts from HT29 cells (lane 1), protein A alone immunoprecipitation (lane 2), immunoprecipitation with nonspecific mouse IgG2a (lane 3), or immunoprecipitation with IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) (lane 4). Western blot was performed using IκBα Antibody #9242.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
- Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
- Gao, H. et al. (2004) Mol. Cell 14, 303-317. Applications: Western Blotting, IP.
- Iwasaki, H. et al. (2011) Nat Immunol 12, 1167-75. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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