Product Pathways - NF-kappaB Signaling
IκB-β Antibody #9248
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 48 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
IκB-β Antibody detects endogenous levels of total IκB-β protein independent of phosphorylation.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the middle of human and mouse IκB-β. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH-3T3 and PC12 cells, untreated or treated with calyculin A #9902 (50 nM for 15 minutes), using IκB-β Antibody.
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκB-α at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκB-α phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
The regulation of IκB-β and IκB-ε is similar to that of IκB-α. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics (9). IKK phosphorylation of IκB-β occurs at Ser19 and Ser23, while IκB-ε can be phosphorylated at Ser18 and Ser22 (10).
- Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
- Hoffmann, A. et al. (2002) Science 298, 1241-1245.
- Shirane, M. et al. (1999) J Biol Chem 274, 28169-28174.
Application References
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Companion Products
- 4921 Phospho-IκB-β (Thr19/Ser23) Antibody (Human Specific)
- 9245 Phospho-IκB-β (Ser19/23) Antibody (Mouse/Rat Specific)
- 4812 IκBα (44D4) Rabbit mAb
- 4814 IκB-α (L35A5) Mouse mAb (Amino-terminal Antigen)
- 9242 IkappaB-alpha Antibody
- 9247 IkappaB-alpha (112B2) Mouse mAb (Carboxy-terminal Antigen)
- 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
- 9246 Phospho-IκBα (Ser32/36) (5A5) Mouse mAb
- 9249 IκB-ε Antibody
- 4924 Phospho-IκB-ε (Ser18/22) Antibody
- 9243 NF-κB Control Cell Extracts
- 9936 NF-κB Pathway Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
