Product Pathways - MAPK Signaling

SAPK/JNK Antibody #9252

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Applications	Reactivity	MW (kDa)	Source
W	H M R Hm B Z	46 JNK1. 54 JNK2/3.	Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  B=Bovine  Z=Zebra Fish
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

SAPK/JNK Antibody detects endogenous levels of total SAPK/JNK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a GST/human JNK2 fusion protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The stress-activated protein kinase/Jun-amino-terminal kinase (SAPK/JNK) is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4-7, which then phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4-7 can be activated by a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family (4). There are three SAPK/JNK genes with further diversification resulting from alternative splicing (3). Active SAPK/JNK dimers can translocate to the nucleus to regulate transcription through its effects on c-Jun, ATF-2 and other transcription factors (3,5).

1. Davis, R.J. (1999) Biochem. Soc. Symp. 64, 1-12.
2. Ichijo, H. (1999) Oncogene 18, 6087-6093.
3. Kyriakis, J.M. and Avruch, J. (2001) Physiol. Rev. 81, 807-869.
4. Kyriakis, J.M. (1999) J. Biol. Chem. 274, 5259-5262.
5. Leppa, S. and Bohmann, D. (1999) Oncogene 18, 6158-6162.
6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.

Application References

• Brouard, S. et al. (2000) Carbon monoxide generated by heme oxygenase 1 suppresses endothelial cell apoptosis. J. Exp. Med. 192, 1015-1026. This article references the use of SAPK/JNK Antibody in the following applications: Western Blotting
• Park, Y. C. et al. (2000) A novel mechanism of TRAF signaling revealed by structural and functional analyses of the TRADD-TRAF2 interaction. Cell 101, 777-787. This article references the use of SAPK/JNK Antibody in the following applications: Western Blotting
• Chen, F. et al. (2001) Opposite effect of NF-kappa B and c-Jun N-terminal kinase on p53-independent GADD45 induction by arsenite. J. Biol. Chem. 276, 11414-11419. This article references the use of SAPK/JNK Antibody in the following applications: Western Blotting
• Hebert, S. S. et al. (2000) The mixed lineage kinase DLK is oligomerized by tissue transglutaminase during apoptosis. J. Biol. Chem. 275, 32482-32490. This article references the use of SAPK/JNK Antibody in the following applications: Western Blotting
• Xiao, G. G. et al. (2003) Use of proteomics to demonstrate a hierarchical oxidative stress response to diesel exhaust particle chemicals in a macrophage cell line. J. Biol. Chem. 278, 50781-50790. This article references the use of SAPK/JNK Antibody in the following applications: Western Blotting
• Banerjee, S. et al. (2002) Murine coronavirus replication-induced p38 mitogen-activated protein kinase activation promotes interleukin-6 production and virus replication in cultured cells. J. Virol. 76, 5937-5948. This article references the use of SAPK/JNK Antibody in the following applications: Western Blotting
• Dai, Y. et al. (2001) Pharmacological inhibitors of the mitogen-activated protein kinase (MAPK) kinase/MAPK cascade interact synergistically with UCN-01 to induce mitochondrial dysfunction and apoptosis in human leukemia cells. Cancer Res. 61, 5106-5115. This article references the use of SAPK/JNK Antibody in the following applications: Western Blotting
• Lee, E. G. et al. (2000) Failure to regulate TNF-induced NF-kappaB and cell death responses in A20-deficient mice. Science 289, 2350-2354. This article references the use of SAPK/JNK Antibody in the following applications: Western Blotting

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