Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor® 647 Conjugate) #9257

Applications Reactivity Sensitivity MW (kDa) Isotype
F H M R Hm Endogenous 46 Phospho-JNK1. 54 Phospho-JNK2/3. Mouse IgG1

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of p46 and p54 SAPK/JNK dually phosphorylated at Thr183 and Tyr185. This antibody does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase.

Source / Purification

Monoclonal antibody is produced by immunizing mice with a synthetic phospho-peptide (KLH coupled) corresponding to residues around Thr183/Tyr185 of human SAPK/JNK. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions with an F/P ratio of 2-6. This antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP1 cells, untreated (blue) or anisomycin-treated (green), using Phospho-SAPK/JNK (T183/Y185) (G9) Mouse mAb (Alexa Fluor®647 Conjugate) (#9257) compared to a nonspecific control antibody (red).

Description

Cell Signaling Technology Antibody conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human and mouse cells. The unconjugated antibody #9255 reacts with, among others, human, mouse, rat and hamster phospho-SAPK/JNK (Thr183/Tyr185). CST expects that Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor® 647 Conjugate) will also recognize phospho-SAPK/JNK (Thr183/Tyr185) in these species.

Application References

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Companion Products

The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 Dye) are covered by pending and issued patents.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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