Product Pathways - MAPK Signaling
Phospho-c-Jun (Ser63) II Antibody #9261
|W IP IF-IC F||H M R Mk Pg||Endogenous||48||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-c-Jun (Ser63) II Antibody detects endogenous levels of c-Jun only when phosphorylated at Ser63. This antibody does not recognize the phosphorylated forms of JunD or JunB.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser63 of human c-Jun. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from anisomycin or UV-treated NIH/3T3 cells, using Phospho-c-Jun (Ser63) II Antibody.
Flow cytometric analysis of Jurkat cells, untreated (blue) or Anisomycin-treated (green), using Phospho-c-Jun (Ser63) II Antibody compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent analysis of HeLa cells, anisomycin-treated (left) or untreated (right) using Phospho-c-Jun (Ser63) II Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
c-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines, and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5), and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival, as well as hypoxia and angiogenesis (8,10). Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis (11,12).
- Jochum, W. et al. (2001) Oncogene 20, 2401-12.
- Davis, R.J. (2000) Cell 103, 239-52.
- Hilberg, F. et al. (1993) Nature 365, 179-81.
- Raivich, G. et al. (2004) Neuron 43, 57-67.
- Behrens, A. et al. (2002) EMBO J 21, 1782-90.
- Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
- Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
- Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
- Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
- Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
- Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
- Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.
- Zhang, S. Q. et al. (2000) Recruitment of the IKK signalosome to the p55 TNF receptor: RIP and A20 bind to NEMO (IKKgamma) upon receptor stimulation. Immunity 12, 301-311. Applications: Western Blot
- Lee, S. A. et al. (2001) Role of ATM in oxidative stress-mediated c-Jun phosphorylation in response to ionizing radiation and CdCl2. J. Biol. Chem. 276, 11783-11790. Applications: Western Blot
- Kleemann, R. et al. (2000) Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1. Nature 408, 211-216. Applications: Western Blot
- de Ruiter, N. D. et al. (2000) Ras-dependent regulation of c-Jun phosphorylation is mediated by the Ral guanine nucleotide exchange factor-Ral pathway. Mol. Cell. Biol. 20, 8480-8488. Applications: Western Blot
- Hou, N. et al. (2008) Endocrinology 149, 1654-65. Applications: Western Blotting
- Tokunaga, F. et al. (2009) Nat Cell Biol 11, 123-32. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.