Cell Signaling Technology

Product Pathways - MAPK Signaling

c-Jun Control Cell Extracts #9263

Description

Nonphosphorylated c-Jun (Ser63) II Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared without treatment, serve as a negative control. Supplied in SDS Sample Buffer.Phosphorylated c-Jun (Ser63) II Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared with UV treatment, serve as a positive control. Supplied in SDS Sample Buffer.

Applications

As controls, we recommend using 15 µl of phosphorylated and nonphosphorylated c-Jun control extracts.

Background

c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress-induced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the amino-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).

  1. Binetruy, B. et al. (1991) Nature 351, 122-127.
  2. Smeal, T. et al. (1991) Nature 354, 494-496.
  3. Derijard, B. et al. (1994) Cell 76, 1025-1037.
  4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.

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