Product Pathways - MAPK Signaling
MKK6 Antibody #9264
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 41 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
MKK6 Antibody detects endogenous levels of total MKK6 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human MKK6. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using MKK6 Antibody.
Background
MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).
- Derijard, B. et al. (1995) Science 267, 682-685.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
- Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
- Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.
Application References
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Companion Products
- 9231 Phospho-MKK3/MKK6 (Ser189/207) Antibody
- 9236 Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb
- 9233 MKK3/MKK6 Control Cell Extracts
- 9232 MKK3 Antibody
- 9238 MKK3b Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
