Product Pathways - Cytoskeletal Signaling
Fascin (D1A8) Rabbit mAb #9269
|W||H M Mk||Endogenous||54||Rabbit IgG|
Reactivity Key: H=Human M=Mouse Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Fascin (D1A8) Rabbit mAb recognizes endogenous levels of total fascin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg201 of human fascin protein.
Fascin is a monomeric, globular protein that plays a central role in regulating the structure and function of the cortical actin cytoskeleton (1). Fascin promotes cross-linkage of parallel actin filaments during the formation of cell protrusions (lamellipodia and filopodia), and therefore plays an important role in regulating cell migration (2). It has been reported that fascin may also regulate filopodia formation by a mechanism independent of its actin-bundling functions (3), though less is known about this mechanism of action. Research studies have shown that increased fascin expression is associated with increased motility and invasiveness of neoplastic cells, including breast, colon, prostate, and esophageal squamous cell carcinomas (4-6). Fascin binds to the armadillo-repeat domain of β-catenin in vitro and in vivo, and has been shown to co-localize with β-catenin and cadherins at the leading edge of migratory cells (7).
- Hoelzle, M.K. and Svitkina, T. (2012) Mol Biol Cell 23, 310-23.
- Adams, J.C. (2004) Curr Opin Cell Biol 16, 590-6.
- Zanet, J. et al. (2012) J Cell Biol 197, 477-86.
- Hashimoto, Y. et al. (2005) Int J Biochem Cell Biol 37, 1787-804.
- Stewart, C.J. et al. (2012) J Clin Pathol 65, 213-7.
- Alam, H. et al. (2012) BMC Cancer 12, 32.
- Tao, Y.S. et al. (1996) J Cell Biol 134, 1271-81.
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