Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

MNDA (3C1) Rat mAb (Alexa Fluor® 488 Conjugate) #9283

Applications Reactivity Sensitivity Isotype
IF-IC F H Endogenous Rat IgG1

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MNDA (3C1) Rat mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total MNDA protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a nuclear extract from human granulocytes.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells (blue) and THP-1 cells (green) using MNDA (3C1) Rat mAb (Alexa Fluor® 488 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of THP-1 cells (left) and Jurkat cells (right) using MNDA (3C1) Rat mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated MNDA (3C1) Rat mAb #3329.

Background

MNDA (myeloid cell nuclear differentiation antigen) is a transcription factor constitutively expressed in peripheral blood granulocytes and monocytes; low expression is also found in a subset of B cells (1,2). MNDA is a member of the interferon (IFN)-regulated 200 family of genes, which contain one or more copies of a partially conserved domain of 200 amino acid residues thought to mediate protein-protein interaction (3). MNDA may play a role in apoptosis and its expression is reduced in myelodysplastic syndromes (MDS) (4). MNDA has been proposed to be a marker for nodal marginal zone lymphoma (5).

  1. Briggs, R.C. et al. (1994) J Cell Biochem 56, 559-67.
  2. Miranda, R.N. et al. (1999) Hum Pathol 30, 1040-9.
  3. Landolfo, S. et al. (1998) Biochimie 80, 721-8.
  4. Briggs, R.C. et al. (2006) Cancer Res 66, 4645-51.
  5. Kanellis, G. et al. (2009) Leukemia 23, 1847-57.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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