Product Pathways - Apoptosis / Autophagy

Phospho-Bad (Ser136) Antibody #9295

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Applications	Reactivity	Source
W	M (H) (R)	Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Bad (Ser136) Antibody detects transfected levels of Bad only when phosphorylated at Ser136. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide corresponding to residues surrounding Ser136 of mouse Bad. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 protein to prevent an association between Bad and Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

1. Yang, E. et al. (1995) Cell 80, 285-291.
2. Zha, J. et al. (1996) Cell 87, 619-628.
3. Datta, S.R. et al. (1997) Cell 91, 231-241.
4. Peso, L. et al. (1997) Science 278, 687-689.
5. Bonni, A. et al. (1999) Science 286, 1358-1362.
6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
7. Harada, H. et al. (1999) Mol. Cell 3, 413-422.
8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.

Application References

• Jamieson, C.A. and Yamamoto, K.R. (2000) Crosstalk pathway for inhibition of glucocorticoid-induced apoptosis by T cell receptor signaling. Proc. Natl. Acad. Sci. USA 97, 7319-7324. This article references the use of Phospho-Bad (Ser136) Antibody in the following applications: Western Blotting
• Laine, J. et al. (2000) The protooncogene TCL1 is an Akt kinase coactivator. Mol. Cell 6, 395-407. This article references the use of Phospho-Bad (Ser136) Antibody in the following applications: Western Blotting
• Yang, C. C. et al. (2003) Bcl-xL mediates a survival mechanism independent of the phosphoinositide 3-kinase/Akt pathway in prostate cancer cells. J. Biol. Chem. 278, 25872-25878. This article references the use of Phospho-Bad (Ser136) Antibody in the following applications: Western Blotting
• Rusinol, A. E. et al. (2004) AKT/protein kinase B regulation of BCL family members during oxysterol-induced apoptosis. J. Biol. Chem. 279, 1392-1399. This article references the use of Phospho-Bad (Ser136) Antibody in the following applications: Western Blotting
• Tan, Y. et al. (1999) p90(RSK) blocks bad-mediated cell death via a protein kinase C-dependent pathway. J. Biol. Chem. 274, 34859-34867. This article references the use of Phospho-Bad (Ser136) Antibody in the following applications: Western Blotting

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