Product Pathways - Apoptosis / Autophagy
Phospho-Bad (Ser112) (7E11) Mouse mAb #9296
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | 23 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Bad (Ser112) (7E11) Mouse mAb detects endogenous levels of Bad only when phosphorylated at serine112. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members.
Source / Purification
Phospho-Bad (Ser112) Antibody detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members.
Western Blotting
Western blot analysis of extracts from COS cells untreated or TPA-treated, using Phospho-Bad (Ser112) (7E11) Mouse mAb (upper) or Bad Antibody #9292 (lower).
Western Blotting
Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A), Bad (S112A/S136A) and treated with TPA or forskolin, using Phospho-Bad (Ser112) (7E11) Mouse mAb (upper) or Bad Antibody #9292 (lower).
Background
Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 protein to prevent an association between Bad and Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).
- Yang, E. et al. (1995) Cell 80, 285-291.
- Zha, J. et al. (1996) Cell 87, 619-628.
- Datta, S.R. et al. (1997) Cell 91, 231-241.
- Peso, L. et al. (1997) Science 278, 687-689.
- Bonni, A. et al. (1999) Science 286, 1358-1362.
- Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
- Harada, H. et al. (1999) Mol. Cell 3, 413-422.
- Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
- Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
- Datta, S. et al. (2000) Mol. Cell 6, 41-51.
Application References
- Avota, E. et al. (2001) Disruption of Akt kinase activation is important for immunosuppression induced by measles virus. Nat. Med. 7 (6), 725-731. This article references the use of Phospho-Bad (Ser112) (7E11) Mouse mAb in the following applications: Western Blotting
- Bieberich, E. et al. (2001) Regulation of apoptosis during neuronal differentiation by ceramide and b-series complex gangliosides. J. Biol. Chem. 276 (48), 44396-44404. This article references the use of Phospho-Bad (Ser112) (7E11) Mouse mAb in the following applications: Western Blotting
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Companion Products
- 9291 Phospho-Bad (Ser112) Antibody
- 9292 Bad Antibody
- 9293 Bad Control Proteins
- 9295 Phospho-Bad (Ser136) Antibody
- 9297 Phospho-Bad (Ser155) Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1026 Phospho-Bad (Ser112) Blocking Peptide
