Product Pathways - Cell Cycle / Checkpoint
Rb Control Proteins #9303
Molecular Weight
76
Description
Nonphosphorylated Rb-C Fusion Protein (5 µg/ml): Rb-C is expressed as a recombinant fusion protein of Rb residues 701?928 and maltose binding protein, serves as a negative control. Supplied in SDS Sample Buffer.Phosphorylated Rb-C Fusion Protein (5 µg/ml): Prepared by in vitro kinase reaction with cdc2, serves as a positive control. Supplied in SDS Sample Buffer.
Directions for Use
As controls, CST recommends using 10 µl (50 ng) of phosphorylated and nonphosphorylated control extracts.
Background
The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
- Sherr, C.J. (1996) Science 274, 1672-1677.
- Nevins, J.R. et al. (1992) Science 258, 424-429.
- Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-790.
- Hu, Q.J. et al. (1990) EMBO J. 9, 1147-1155.
- Knudsen, E.S. and Wang, J.Y. (1997) Mol. Cell. Biol. 17, 5771-5783.
- Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753-761.
- Connell-Crowley, L. et al. (1997) Mol. Cell. Biol. 8, 287-301.
- Kitagawa, M. et al. (1996) EMBO J. 15, 7060-7069.
- Geng, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 194-199.
Application References
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Companion Products
- 9301 Phospho-Rb (Ser795) Antibody
- 9307 Phospho-Rb (Ser780) Antibody
- 9308 Phospho-Rb (Ser807/811) Antibody
- 6022 Rb-C Fusion Protein
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9309 Rb (4H1) Mouse mAb
Western Blots: CST recommends using 10 µl (50 ng) of phosphorylated and nonphosphorylated control extracts as controls.
