Product Pathways - Cell Cycle / Checkpoint
Phospho-Rb (Ser780) Antibody #9307
PhosphoSitePlus® protein, site, and accession data: Rb
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP E-P | H R Mk | Endogenous | 110 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
E-P=ELISA (Peptide)
Reactivity Key:
H=Human
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-Rb (Ser780) Antibody detects endogenous levels of Rb only when phosphorylated at Ser780. The antibody does not cross-react with Rb phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Rb. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from human fibroblasts synchronized by serum deprivation, using Phospho-Rb (Ser780) Antibody. Cells were synchronized for 24 hours then released by addition of serum and harvested at the times indicated. Cell cycle progression was verified by cyclin analysis and FACS. (Provided by John Boylan, Dupont/Merck, Delaware.)
Background
The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
- Sherr, C.J. (1996) Science 274, 1672-7.
- Nevins, J.R. (1992) Science 258, 424-9.
- Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
- Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
- Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
- Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
- Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
- Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
- Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
Application References
- Chiariello, M. et al. (2000) Regulation of cyclin-dependent kinase (Cdk) 2 Thr-160 phosphorylation and activity by mitogen-activated protein kinase in late G1 phase. Biochem. J. 349, 869-876. Applications: Western Blotting
- Fujimoto, K. et al. (2000) Inhibition of pRb phosphorylation and cell cycle progression by an antennapedia-p16(INK4A) fusion peptide in pancreatic cancer cells. Cancer Lett. 159, 151-158. Applications: Western Blotting
- Huang, H. et al. (2004) Androgens repress Bcl-2 expression via activation of the retinoblastoma (RB) protein in prostate cancer cells. Oncogene 23, 2161-2176. Applications: Western Blotting
- Feng, L. X. et al. (2000) Stem cell factor/c-kit up-regulates cyclin D3 and promotes cell cycle progression via the phosphoinositide 3-kinase/p70S6 kinase pathway in Spermatogonia. J. Biol. Chem. 275, 25572-25576. Applications: Western Blotting
- Dupont, J. et al. (2000) The potentiation of estrogen on insulin-like growth factor I action in MCF-7 human breast cancer cells includes cell cycle components. J. Biol. Chem. 275, 35893-35901. Applications: Western Blotting
- Faenza, I. et al. (2000) A role for nuclear phospholipase c beta 1 in cell cycle control. J. Biol. Chem. 275, 30520-30524. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9301 Phospho-Rb (Ser795) Antibody
- 9308 Phospho-Rb (Ser807/811) Antibody
- 2181 Phospho-Rb (Ser608) Antibody
- 9309 Rb (4H1) Mouse mAb
- 9917 Cell Cycle/Checkpoint Antibody Sampler Kit
- 9306 PhosphoPlus® Rb (Ser780, Ser807/811) Antibody Kit
- 9969 Rb Antibody Sampler Kit
- 9303 Rb Control Proteins
- 1142 Rb (Ser780) Biotinylated Peptide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3590 Phospho-Rb (Ser780) (C84F6) Rabbit mAb
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
