Product Pathways - Tyrosine Kinase/ Adaptors
Tyk2 Antibody #9312
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H | Endogenous | 140 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Tyk2 Antibody detects endogenous levels of total Tyk2. No cross-reactivity was detected with other family members at physiological conditions.
Source / Purification
Polyclonal antibodies are prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding serine 819 of human Tyk2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Raji and Jurkat cells, using Tyk2 Antibody.
IF-IC
Immunofluorescent analysis showing cytoplasmic staining of MCF-7 cells, using Tyk2 Antibody.
Background
Tyk2, a member of the Jak family of protein tyrosine kinases, associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and interferon-alpha and beta (1-3). Following ligand binding, Tyk2 is activated by phosphorylation on Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-beta signaling cascade (5).
- Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-3334.
- Stahl, N. et al. (1994) Science 263, 92-95.
- Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Grauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.
- Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-32511.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
