Cell Signaling Technology

Product Pathways - Metabolism

Tyrosinase (T311) Mouse mAb (IHC Specific) #9319

Applications Reactivity Sensitivity MW (kDa) Isotype
IHC-P H Endogenous 70-80 Mouse IgG2a

Applications Key:  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

Tyrosinase (T311) Mouse mAb detects endogenous levels of total tyrosinase protein.

Source / Purification

Tyrosinase (T311) Mouse mAb is produced by immunizing animals with a purified recombinant tyrosinase.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of human colon tissue using Tyrosinase (T311) Mouse mAb (IHC Specific). Note the lack of staining in the tyrosinase negative tissue.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of human melanoma tissue using Tyrosinase (T311) Mouse mAb (IHC Specific).

Background

Tyrosinase, found in the membrane of melanosomes (1), is a key enzyme in the biosynthesis of melanin pigments (1,2). It is a melanocyte differentiation antigen and is expressed in normal melanocytes and malignant melanomas (1,3). Tyrosinase is implicated to be an antigen target for melanoma vaccines (1).

  1. Chen, Y.T. et al. (1995) Proc Natl Acad Sci USA 92, 8125-9.
  2. Kwon, B.S. (1993) J Invest Dermatol 100, 134S-140S.
  3. Kwon, B.S. et al. (1987) Proc Natl Acad Sci USA 84, 7473-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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