Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Src Family Antibody Sampler Kit #9320

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
Src (32G6) Rabbit mAb #2123 40 µl W IP H M R (C) 60 Rabbit IgG
Csk (C74C1) Rabbit mAb #4980 40 µl W H M R Mk Pg 50 Rabbit IgG
Fyn Antibody #4023 40 µl W H M 59 Rabbit
Lck (D88) XP® Rabbit mAb #2984 40 µl W IHC-P H 56 Rabbit IgG
Lyn (C13F9) Rabbit mAb #2796 40 µl W IP IHC-P H M R Mk 56 Rabbit IgG
Yes Antibody #3201 40 µl W IP H Mk B 60 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  B=Bovine  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the Src Family Antibody Sampler Kit detects endogenous levels of its target protein and does not cross-react with other family members.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Src (32G6) Rabbit mAb #2123.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lyn (C13F9) Rabbit mAb #2796.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos and Jurkat cells using LcK (D88) XP® Rabbit mAb #2984.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Yes Antibody #3201.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Fyn Antibody #4023.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Csk (C74C1) Rabbit mAb #4980.


Description

The Src Family Antibody Sampler Kit provides an economical means of evaluating total levels of Src family member proteins. The kit contains enough primary and secondary antibody to perform four western blots with each antibody.

Source / Purification

Src (32G6) Monoclonal antibody is produced by immunizing animals with a GST-Src fusion protein containing amino acid residues 1-110 of human Src. The remaining monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val399 of human Csk, Thr50 of human Lck, and the amino-terminal sequence of human Lyn. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser25 of human Fyn and the amino terminus of human Yes. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

The kinase Fyn plays a role in T-cell receptor signaling and also helps in memory formation and adhesion mediated signaling (3,4). Lymphocyte specific kinase Lck is essential in the differentiation and activation of T-cells (3). Hematopoietic Lyn kinase, also phosphorylated by Csk, is involved in the regulation of B-cell function, migration and development (5). The ubiquitously expressed kinase Yes acts downstream of several different cell surface receptors, including G-protein-coupled receptors, and is involved in the regulation of angiogenesis, the cell cycle and cell adhesion (6,7).

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.
  3. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
  4. Isosaka, T. et al. (2008) Eur. J. Neurosci. 28, 973-981.
  5. Seo, S. et al. (2001) J. Immunol. 166, 3710-3716.
  6. Summy, J.M. et al. (2003) J. Cell Sci. 116, 2585-2598.
  7. Werdich, X.Q. and Penn, J.S. (2005) Angiogenesis 8, 315-326.

Application References

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Protocols

Companion Products

Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and/or 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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