Product Pathways - Tyrosine Kinase / Adaptors
Phospho-Tyk2 (Tyr1054/1055) Antibody #9321
|W||H (M) (R)||Endogenous||140||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-Tyk2 (Tyr1054/1055) Antibody detects endogenous levels of Tyk2 only when phosphorylated at tyrosine1054/1055. The antibody does not cross-react with Tyk2 phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1054/1055 of human Tyk2. Antibodies are purified by protein A and peptide affinity chromatography.
Tyk2, a member of the Jak family of protein tyrosine kinases, associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and interferon-alpha and beta (1-3). Following ligand binding, Tyk2 is activated by phosphorylation on Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-beta signaling cascade (5).
- Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-3334.
- Stahl, N. et al. (1994) Science 263, 92-95.
- Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Grauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.
- Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-32511.
- Komatsu, T. et al. (2000) Sendai virus blocks alpha interferon signaling to signal transducers and activators of transcription. J. Virol. 74, 2477-2480. Applications: Western Blotting
- Lejeune, D. et al. (2002) IL-22 activates the JAK/STAT, ERK, JNK and p38 MAP kinase pathways in a rat hepatoma cell line: shared and distinct pathways from IL-10. J. Biol. Chem. 277 (37), 33676-33682. Applications: Western Blotting
- Fiedler, J. et al. (2012) Haematologica 97, 73-81. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.