Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-Tyk2 (Tyr1054/1055) Antibody #9321
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) | Endogenous | 140 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Tyk2 (Tyr1054/1055) Antibody detects endogenous levels of Tyk2 only when phosphorylated at tyrosine1054/1055. The antibody does not cross-react with Tyk2 phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr1054/1055 of human Tyk2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or interferon-alpha treated for the indicated times, using Phospho-Tyk2 (Tyr1054/1055) Antibody (upper) or Tyk2 antibody (lower).
Background
Tyk2, a member of the Jak family of protein tyrosine kinases, associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and interferon-alpha and beta (1-3). Following ligand binding, Tyk2 is activated by phosphorylation on Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-beta signaling cascade (5).
- Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-3334.
- Stahl, N. et al. (1994) Science 263, 92-95.
- Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Grauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.
- Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-32511.
Application References
- Komatsu, T. et al. (2000) Sendai virus blocks alpha interferon signaling to signal transducers and activators of transcription. J. Virol. 74, 2477-2480. This article references the use of Phospho-Tyk2 (Tyr1054/1055) Antibody in the following applications: Western Blotting
- Lejeune, D. et al. (2002) IL-22 activates the JAK/STAT, ERK, JNK and p38 MAP kinase pathways in a rat hepatoma cell line: shared and distinct pathways from IL-10. J. Biol. Chem. 277 (37), 33676-33682. This article references the use of Phospho-Tyk2 (Tyr1054/1055) Antibody in the following applications: Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
