Product Pathways - Tyrosine Kinase / Adaptors
Phospho-Tyk2 (Tyr1054/1055) Antibody #9321
PhosphoSitePlus® protein, site, and accession data: Tyk2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) | Endogenous | 140 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9321:
- Western Blotting
Specificity / Sensitivity
Phospho-Tyk2 (Tyr1054/1055) Antibody detects endogenous levels of Tyk2 only when phosphorylated at tyrosine1054/1055. The antibody does not cross-react with Tyk2 phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1054/1055 of human Tyk2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or interferon-alpha treated for the indicated times, using Phospho-Tyk2 (Tyr1054/1055) Antibody (upper) or Tyk2 antibody (lower).
Background
Tyk2, a member of the Jak family of protein tyrosine kinases, associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and interferon-alpha and beta (1-3). Following ligand binding, Tyk2 is activated by phosphorylation on Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-beta signaling cascade (5).
- Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-3334.
- Stahl, N. et al. (1994) Science 263, 92-95.
- Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Grauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.
- Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-32511.
Application References
- Komatsu, T. et al. (2000) Sendai virus blocks alpha interferon signaling to signal transducers and activators of transcription. J. Virol. 74, 2477-2480. Applications: Western Blotting
- Lejeune, D. et al. (2002) IL-22 activates the JAK/STAT, ERK, JNK and p38 MAP kinase pathways in a rat hepatoma cell line: shared and distinct pathways from IL-10. J. Biol. Chem. 277 (37), 33676-33682. Applications: Western Blotting
- Fiedler, J. et al. (2012) Haematologica 97, 73-81. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9312 Tyk2 Antibody
- 9131 Phospho-Stat3 (Tyr705) Antibody
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- 9351 Phospho-Stat5 (Tyr694) Antibody
- 9361 Phospho-Stat6 (Tyr641) Antibody
- 9914 Phospho-Stat Antibody Sampler Kit
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
