Product Pathways - PI3K / Akt Signaling
Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb #9322
Have you tried your application using our XP® monoclonal antibodies? Try product: 5558
PhosphoSitePlus® protein, site, and accession data: GSK3B
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 46 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb detects endogenous levels of GSK-3-beta only when phosphorylated at Ser9.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser9 of human GSK-3-beta.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, lambda-phosphatase or PDGF-treated, using Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb (upper) or GSK-3-beta (27C10) Rabbit mAb #9315 (lower).
Western Blotting
Western blot analysis of extracts from PDGF-treated wild-type, GSK-3-alpha (-/-) and GSK-3-beta (-/-) mouse embryonic fibroblasts (MEFs) using Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb (upper) and GSK-3-alpha/beta Antibody (lower). (MEF wild-type, GSK-3-alpha (-/-) and GSK-3-beta (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
- Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
- Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
- Cross, D.A. et al. (1995) Nature 378, 785-789.
- Nusse, R. (1997) Cell 89, 321-323.
- Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.
Application References
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Companion Products
- 9316 Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb
- 9327 Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred)
- 9338 GSK-3α Antibody
- 9315 GSK-3β (27C10) Rabbit mAb
- 9270 PhosphoPlus® Akt (Ser473) Antibody Kit
- 9916 Phospho-Akt Pathway Antibody Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 8213 PhosphoPlus® GSK-3β (Ser9) Antibody Duet
For Research Use Only. Not For Use In Diagnostic Procedures.
