Product Pathways - MAPK Signaling
Phospho-RSK2 (Tyr529) Antibody #9324
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H Mk | Endogenous | 90 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-RSK2 (Tyr529) Antibody detects endogenous RSK2 protein only when phosphorylated at Tyr529. This antibody cross-reacts with some tyrosine phosphorylated proteins including EGFR.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Tyr529 of human RSK2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from untreated or FGF-treated SK-N-MC cells, using Phospho-RSK2 (Tyr529) Antibody (upper) or RSK2 Antibody #9340 (lower).
Background
The 90 kDa ribosomal S6 kinases (RSK1?4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a C-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363 and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones and neurotransmitters (3).
Phosphorylation of RSK2 on Tyr529 was identified at Cell Signaling Technology (CST) using PhosphoScan®, our LC-MS/MS platform for phosphorylation site discovery. This study identifies RSK2 as a key substrate of FGFR3 in human t(4;14)-positive multiple myeloma (MM) cells. Constitutively active FGFR3 directly phosphorylates RSK2 on Tyr529, which primes RSK2 for activation by ERK1/2 (4). RSK2 can also be phosphorylated at Tyr529 by Src family tyrosine kinases Src and Fyn in response to EGF stimulation (5). Mutations in the RSK2 gene are associated with Coffin-Lowry syndrome (CLS), an X-linked disorder characterized by mental retardation and the presence of characteristic facial anomalies (6).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Kang, S. et al. (2007) Cancer Cell 12, 201-14.
- Kang, S. et al. (2007) J Biol Chem Submitted for Publication
- Delaunoy, J.P. et al. (2006) Clin Genet 70, 161-6.
Application References
- Kang, S. et al. (2007) Cancer Cell 12, 201-14. This article references the use of Phospho-RSK2 (Tyr529) Antibody in the following applications: Western Blotting
- Kang, S. et al. (2007) J Biol Chem Submitted for publication. This article references the use of Phospho-RSK2 (Tyr529) Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9335 Phospho-p90RSK (Ser380) (9D9) Rabbit mAb
- 9341 Phospho-p90RSK (Ser380) Antibody
- 9344 Phospho-p90RSK (Thr359/Ser363) Antibody
- 9346 Phospho-p90RSK (Thr573) Antibody
- 9348 Phospho-RSK3 (Thr356/Ser360) Antibody
- 9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb
- 9347 RSK1/RSK2/RSK3 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9333 RSK1 Antibody
- 9340 RSK2 Antibody
- 9343 RSK3 Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
