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GSK-3β-Tag Antibody #9325

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	All	Endogenous	n/a	Rabbit

Applications Key: W=Western Blotting
Reactivity Key: All=All species expected

Specificity / Sensitivity

GSK-3β-Tag Antibody detects transfected levels of GSK-3β-tagged proteins as well as endogenous GSK-3β (46 kDa).

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic GSK-3β-Tag peptide (KLH-coupled). Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HeLa cells, control (lane 1) or infected with YopE129-GSK-3β (lane 2 and 3), using GSK-3β-Tag Antibody (lysates kindly provided by Dr. Gregory Plano, University of Miami).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Short phosphorylatable peptide tags, such as the GSK-3β-tag, can be used to determine translocation of effector proteins in type III secretion systems (1).

Hohlfeld, S. et al. (2006) Mol. Microbiol. 59, 1624-1637.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.