Product Pathways - Complementary Reagents
GSK-3β-Tag Antibody #9325
PhosphoSitePlus® protein, site, and accession data: GSK3B
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | All | Endogenous | n/a | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 9325:
- Western Blotting
Specificity / Sensitivity
GSK-3β-Tag Antibody detects transfected levels of GSK-3β-tagged proteins as well as endogenous GSK-3β (46 kDa).
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic GSK-3β-Tag peptide. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, control (lane 1) or infected with YopE129-GSK-3β (lane 2 and 3), using GSK-3β-Tag Antibody (lysates kindly provided by Dr. Gregory Plano, University of Miami).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Short phosphorylatable peptide tags, such as the GSK-3β-tag, can be used to determine translocation of effector proteins in type III secretion systems (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
