Cell Signaling Technology

Product Pathways - MAPK Signaling

p90RSK Antibody Sampler Kit #9326

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
Phospho-p90RSK (Ser380) (9D9) Rabbit mAb #9335 40 µl W H M R Mk (C) 90 Rabbit IgG
Phospho-p90RSK (Thr359/Ser363) Antibody #9344 40 µl W IP H M R Mk 90 Rabbit
Phospho-p90RSK (Thr573) Antibody #9346 40 µl W IF-IC H M R (Hm) (C) (X) (Z) 90 Rabbit
RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 40 µl W IP IF-IC H M R Mk GP 90 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  X=Xenopus  Z=Zebrafish  GP=Guinea Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

RSK1/RSK2/RSK3 (32D7) Rabbit mAb detects endogenous levels of total RSK1-3 proteins. Each activation state monoclonal antibody recognizes its specific target; some cross-reactivity with related phosphorylated RSK proteins may occur.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or treated with TPA #9905, using Phospho-p90RSK (Ser380) (9D9) Rabbit mAb #9335.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and C6 cells, untreated or treated with TPA #9905 (200 nM), using Phospho-p90RSK (Thr359/Ser363) Antibody #9344 (upper) or control RSK1/RSK2/RSK3 Rabbit mAb #9355 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with TPA #9905 (200 nM), using Phospho-p90RSK (Thr573) Antibody #9346 (upper) or RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, L-929 and AR42J cells using RSK1/RSK2/RSK3 (32D7) #9355 Rabbit mAb.

Description

The p90RSK Antibody Sampler Kit provides an economical means of evaluating levels of p90RSK protein phosphorylated at specific sites, as well as total p90RSK protein levels. The kit contains enough primary and secondary antibody to perform four Western blot experiments.

Source / Purification

The phospho-specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr359/Ser363 of rat RSK1 and Thr573 of human p90RSK. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. The rabbit monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser380 of human p90RSK and a nonphosphorylated peptide surrounding Asp659 of human p90RSK for total RSK1/RSK2/RSK3.

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

Application References

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Protocols

Companion Products

Rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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