Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

c-Oncogene Antibody Sampler Kit #9328

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
c-Abl Antibody #2862 40 µl W IP IF-IC H M R 135 (c-Abl); 210 (Bcr-Abl) Rabbit
c-Fos (9F6) Rabbit mAb #2250 40 µl W IF-IC F ChIP H M R (Hm) (B) (Pg) 62 Rabbit IgG
c-Jun (60A8) Rabbit mAb #9165 40 µl W IP IHC-P IHC-F IF-IC ChIP H M R Mk 43, 48 Rabbit IgG
c-Kit (D13A2) XP® Rabbit mAb #3074 40 µl W IP IF-IC H M 120 and 145 Rabbit
c-Myc (D84C12) XP® Rabbit mAb #5605 40 µl W IP IF-IC F H M R (Mk) (Dg) (Pg) 57-65 Rabbit IgG
c-Raf Antibody #9422 40 µl W H M R Mk 65 to 75 Rabbit
Ras (27H5) Rabbit mAb #3339 40 µl W H M R Mk Dm Sc 21 Rabbit IgG
c-Rel Antibody #4727 40 µl W IP IHC-P IF-IC F H Mk 78 Rabbit
Src (36D10) Rabbit mAb #2109 40 µl W IP IHC-P IHC-F IF-F IF-IC F H M R Hm Mk B Pg (C) 60 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry  ChIP=Chromatin IP
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Dm=D. melanogaster  B=Bovine  Dg=Dog  Pg=Pig  Sc=S. cerevisiae
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Unless otherwise indicated, each antibody in the c-Oncogene Antibody Sampler Kit detects endogenous levels of total target protein and does not cross-react with related proteins. c-Jun (60A8) Rabbit mAb detects endogenous levels of total c-Jun protein, regardless of phosphorylation state. Ras (27H5) Rabbit mAb detects endogenous levels of total K-Ras, H-Ras and N-Ras proteins. Src (36D10) Rabbit mAb detects endogenous levels of Src proteins and may cross-react with other Src family members. The c-Myc (D84C12) Rabbit mAb detects endogenous levels of total c-Myc protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using c-Myc (D84C12) XP® Rabbit mAb #5605.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Src (36D10) Rabbit mAb #2109.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and H-4-II-E cells, serum-starved overnight and TPA-stimulated for 4 hours, using c-Fos (9F6) Rabbit mAb #2250.


Western Blotting

Western Blotting

Western blot analysis of extracts from LNCap, TT and K-562 cells using c-Abl Antibody #2862.

Western Blotting

Western Blotting

Western blot analysis of extracts from H526 cells using c-Kit (D132A) XP® Rabbit mAb #3074.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T, C2C12 and C6 cells using Ras (27H5) Rabbit mAb #3339.


Western Blotting

Western Blotting

Western blot analysis of extracts from K-562, Raji and 293 cells using c-Rel Antibody #4727.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and SK-N-MC cells, untreated or UV-treated, using c-Jun (60A8) Rabbit mAb #9165.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, C2C12 and PC-12 cells, untreated or TPA-treated (200 nM for 30 minutes), using c-Raf Antibody #9422.


Description

The c-Oncogene Antibody Sampler Kit provides an economical means of evaluating total levels of various oncogenic proteins. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human c-Rel, a region surrounding Pro302 of human c-Raf, and corresponding to the sequence close to the carboxy-terminus of human c-Abl. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human Src, residues near the amino terminus of human K-Ras, from the amino-terminal sequence of human c-Jun, from the sequence of human c-Fos, residues near the amino terminus of c-Myc and corresponding to the residues surrounding Tyr703 of human c-Kit.

Background

The regulation of cell growth, differentiation and programmed death is coordinated by several sets of proteins that comprise essential signal transduction pathways. Many of these key regulatory proteins are encoded by proto-oncogenes, which can be activated (altered) to change the typical cell program to one of abnormal cell growth and unregulated development. Proteins encoded by proto-oncogenes include growth factors and other ligands, receptor proteins, tyrosine kinases, various regulatory proteins (i.e. GTPases) and transcription factors. Together these proteins comprise the basic elements of cell signaling pathways; altered expression or mutation of one or more of these components can lead to oncogenic growth (reviewed in 1).Non-receptor (i.e. cytoplasmic, nuclear) tyrosine kinases such as c-Abl and Src play key roles in the regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (2,3). Alteration of the corresponding c-Abl and Src proto-oncogenes is associated with oncogenesis; Abl1-BCR gene translocations result in chronic myelogenous leukemia (CML) while constitutively active Src is seen in some patients with colon cancer and altered Src expression is seen in a wide array of cancers (2,4). Regulation of Raf tyrosine kinase by Ras GTPase controls downstream kinases in the MEK/MAPK signaling pathway (5). Activation of the Ras and Raf proto-oncogenes are common in human cancers and both proteins are seen as potential therapeutic targets (6). The receptor tyrosine kinase c-Kit plays a critical role in activation and growth of hematopoietic stem cells (7); mutations that inhibit c-Kit kinase activity are associated with a variety of developmental disorders while mutations producing constitutively active c-Kit can result in mastocytosis and gastrointestinal stromal tumors (8). The alteration of key transcription factors such as c-Fos, c-Jun, c-Myc and c-Rel that are normally responsible for regulating cell and tissue growth, differentiation and the inflammation/immune response, can also result in unregulated, oncogenic cell growth (9-12).

  1. Croce, C.M. (2008) N Engl J Med 358, 502-11.
  2. Wang, J.Y. (2000) Oncogene 19, 5643-50.
  3. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
  4. Dehm, S.M. and Bonham, K. (2004) Biochem Cell Biol 82, 263-74.
  5. Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83.
  6. Stites, E.C. et al. (2007) Science 318, 463-7.
  7. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
  8. Nocka, K. et al. (1990) EMBO J 9, 1805-13.
  9. Milde-Langosch, K. (2005) Eur J Cancer 41, 2449-61.
  10. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
  11. Yokota, J. et al. (1986) Science 231, 261-5.
  12. Rayet, B. and Gélinas, C. (1999) Oncogene 18, 6938-47.

Application References

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Protocols

Companion Products

Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and/or U.S.S.N. 11/476,277) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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