Product Pathways - MAPK Signaling
MAPKAPK-2 Antibody Sampler Kit #9329
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| MAPKAPK-2 Antibody #3042 | 40 µl | W IP F | H M R Mk (Hm) | 47, 49 (Phosphorylated). | Rabbit |
| Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 | 40 µl | W IHC-P IF-IC F | H M R Mk | 49 | Rabbit IgG |
| Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316 | 40 µl | W | H M R Mk | 49 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the MAPKAPK-2 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. MAPKAPK-2 Antibody does not cross-react with MAPKAPK-3 or -5.
Western Blotting
Western blot analysis of extracts from HeLa and COS cells, untreated or UV and TPA-treated, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 (upper) and MAPKAPK-2 Antibody #3042 (lower).
Western Blotting
Western blot analysis of extracts from HeLa and COS cells, untreated or UV-treated, using MAPKAPK-2 Antibody #3042.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or UV and TPA-treated, using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316 (upper) and MAPKAPK-2 Antibody #3042 (lower).
Description
The MAPKAPK-2 Antibody Sampler Kit provides an economical means of evaluating levels of MAPKAPK-2 protein phosphorylated at specific sites, as well as total MAPKAPK-2 protein levels. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal region of human MAPKAPK-2. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr222 and Thr334 of human MAPKAPK-2.
Background
In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).
Application References
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Protocols
- 3007:
- Flow, IHC / Paraffin, Immunofluorescence, Western Blotting
- 3042:
- Flow, Immunoprecipitation, Western Blotting
- 3316:
- Western Blotting
Companion Products
- 3043 MAPKAPK-3 Antibody
- 4320 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4338 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
