Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

FAK Antibody Sampler Kit #9330

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
Phospho-FAK (Tyr397) Antibody #3283 40 µl W H M R Hm Pg 125 Rabbit
Phospho-FAK (Tyr576/577) Antibody #3281 40 µl W IP H M R (C) (X) 125 Rabbit
Phospho-FAK (Tyr925) Antibody #3284 40 µl W IP IF-IC H (M) (R) (C) 125 Rabbit
FAK Antibody #3285 40 µl W IP IHC-P H M R Mk B Pg (C) 125 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  X=Xenopus  B=Bovine  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the FAK Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross react with other proteins. Activation state antibodies recognize target proteins only when phosphorylated at the indicated residue. Phospho-FAK (Tyr397) Antibody may cross-react with overexpressed tyrosine phosphorylated EGF receptors.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr576/577) Antibody #3281 (upper) or FAK Antibody #3285 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untransfected or transfected with activated Src, using Phospho-FAK (Tyr397) Antibody #3283 (upper) or FAK Antibody #3285 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr925) Antibody #3284 (upper) or FAK Antibody #3285 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FAK Antibody #3285.

Description

The FAK Antibody Sampler Kit provides an economical means of evaluating total FAK protein levels as well as FAK phosphorylated at specific sites. The kit contains enough primary and secondary antibody to perform four western blots with each antibody.

Source / Purification

Activation state polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr397, Tyr576/577 and Tyr 925 of human FAK protein. FAK Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 710 of human FAK protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

  1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613.
  2. Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
  3. Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155.
  4. Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
  5. Zhang, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 9021-9026.
  6. Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.
  7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

Application References

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Protocols

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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