Product Pathways - MAPK Signaling
RSK1 Antibody #9333
|W||H M R Mk||Endogenous||90||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
RSK1 Antibody detects endogenous levels of RSK1. It does not cross-react with the RSK2 or RSK3 isoforms.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu718 of human RSK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of purified RSK1, RSK2, and RSK3 proteins using RSK1 Antibody (upper), RSK2 Antibody #9340 (middle) or RSK3 Antibody #9343 (lower).
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).
PI3K-induced activation of RSK1 is mediated by the Ser/Thr kinase mTOR (mammalian target of rapamycin) (4,5). This activation of RSK1 selectively increases the translation of mRNA transcripts containing a tract of pyrimidine (TOP) motif. An association between RSK1 and specific PKA subunits depends upon RSK1 activation state and determines both intracellular localization and specific activity of the kinase (6). Evidence from animal models suggests that RSK1 is a key regulator of glucose homeostasis and cell size (7).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
- Um, S.H. et al. (2006) Cell Metab 3, 393-402.
- Chaturvedi, D. et al. (2006) Mol Cell Biol 26, 4586-600.
- Ruvinsky, I. et al. (2005) Genes Dev 19, 2199-211.
- Fonseca, B.D. et al. (2011) J Biol Chem 286, 27111-22. Applications: Western Blotting
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- 9326 p90RSK Antibody Sampler Kit
- 3556 Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb
- 9335 Phospho-p90RSK (Ser380) (9D9) Rabbit mAb
- 9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb
- 9340 RSK2 Antibody
- 9343 RSK3 Antibody
- 9341 Phospho-p90RSK (Ser380) Antibody
- 9344 Phospho-p90RSK (Thr359/Ser363) Antibody
- 9346 Phospho-p90RSK (Thr573) Antibody
- 9347 RSK1/RSK2/RSK3 Antibody
- 9348 Phospho-RSK3 (Thr356/Ser360) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.