Cell Signaling Technology

Product Pathways - MAPK Signaling

RSK1 Antibody #9333

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 90 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RSK1 Antibody detects endogenous levels of RSK1. It does not cross-react with the RSK2 or RSK3 isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu718 of human RSK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of purified RSK1, RSK2, and RSK3 proteins using RSK1 Antibody (upper), RSK2 Antibody #9340 (middle) or RSK3 Antibody #9343 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and COS cells using RSK1 Antibody.

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

PI3K-induced activation of RSK1 is mediated by the Ser/Thr kinase mTOR (mammalian target of rapamycin) (4,5). This activation of RSK1 selectively increases the translation of mRNA transcripts containing a tract of pyrimidine (TOP) motif. An association between RSK1 and specific PKA subunits depends upon RSK1 activation state and determines both intracellular localization and specific activity of the kinase (6). Evidence from animal models suggests that RSK1 is a key regulator of glucose homeostasis and cell size (7).

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
  4. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
  5. Um, S.H. et al. (2006) Cell Metab 3, 393-402.
  6. Chaturvedi, D. et al. (2006) Mol Cell Biol 26, 4586-600.
  7. Ruvinsky, I. et al. (2005) Genes Dev 19, 2199-211.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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