Product Pathways - Akt Signaling

Phospho-GSK-3β (Ser9) Antibody #9336

Applications	Reactivity	MW (kDa)	Source
W	H M R Mk (Z)	46	Rabbit

Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Z=Zebra Fish
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-GSK-3beta (Ser9) Antibody detects endogenous levels of GSK-3β only when phosphorylated at serine 9. The antibody may cross-react weakly with the phosphorylated form of GSK-3α due to high sequence homology.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) derived from the sequence of human GSK-3β. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF treated for the indicated times, using Phospho-GSK-3beta (Ser9) Antibody (upper) or GSK-3beta Antibody #9332 (lower).

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulated glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

Application References

Crowder, R.J. and Freeman, R.S. (2000) Glycogen synthase kinase-3beta activity is critical for neuronal death caused by inhibiting phosphatidylinositol 3-kinase or Akt but not for death caused by nerve growth factor withdrawal. J. Biol. Chem. 275, 34266-34271. This article references the use of Phospho-GSK-3β (Ser9) Antibody in the following applications: Western Blotting
Fasshauer, M. et al. (2000) Essential role of insulin receptor substrate-2 in insulin stimulation of Glut4 translocation and glucose uptake in brown adipocytes. J. Biol. Chem. 275, 25494-25501. This article references the use of Phospho-GSK-3β (Ser9) Antibody in the following applications: Western Blotting
Ohteki, T. et al. (2000) Negative regulation of T cell proliferation and C278 interleukin 2 production by the serine threonine kinase GSK-3. J. Exp. Med. 192, 991-104. This article references the use of Phospho-GSK-3β (Ser9) Antibody in the following applications: Western Blotting
Okano, J. et al. (2000) Akt/protein kinase B isoforms are differentially regulated by epidermal growth factor stimulation. J. Biol. Chem. 275, 30934-30942. This article references the use of Phospho-GSK-3β (Ser9) Antibody in the following applications: Western Blotting
Nakamura, N. et al. (2000) Forkhead transcription factors are critical effectors of cell death and cell cycle arrest downstream of PTEN. Mol. Cell. Biol. 20, 8969-8982. This article references the use of Phospho-GSK-3β (Ser9) Antibody in the following applications: Western Blotting
Morfini, G. et al. (2002) Glycogen Synthase Kinase 3 phosphorylates kinesin light chains and negatively regulates kinesin-based motility. EMBO 21 (3), 281-293. This article references the use of Phospho-GSK-3β (Ser9) Antibody in the following applications: IC-IF
Vitari, A.C. et al. (2004) Biochem J 378, 257-68. This article references the use of Phospho-GSK-3β (Ser9) Antibody in the following applications: Western Blotting

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Companion Products

9270 PhosphoPlus® Akt (Ser473) Antibody Kit
9331 Phospho-GSK-3α/β (Ser21/9) Antibody
9916 Phospho-Akt Pathway Sampler Kit
7074 Anti-rabbit IgG, HRP-linked Antibody
7720 Prestained Protein Marker, Broad Range (Premixed Format)
7727 Biotinylated Protein Ladder Detection Pack
7003 20X LumiGLO^® Reagent and 20X Peroxide
9315 GSK-3β (27C10) Rabbit mAb