Product Pathways - Akt Signaling
Phospho-GSK-3α (Ser21) (46H12) Mouse mAb #9337
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk Z | Endogenous | 51 | Mouse IgG2b |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Z=Zebra Fish
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-GSK-3alpha (Ser21) (46H12) Mouse mAb detects endogenous levels of GSK-3alpha only when phosphorylated at serine 21.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser21 of human GSK-3alpha.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, control and serum treated, using Phospho-GSK-3alpha (Ser21) (46H12) Mouse mAb (top), Phospho-GSK-3alpha/beta (Ser21/9) Antibody #9331 (middle) and GSK-3beta Antibody (bottom).
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is an ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
- Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
- Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
- Cross, D.A. et al. (1995) Nature 378, 785-789.
- Nusse, R. (1997) Cell 89, 321-323.
- Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9331 Phospho-GSK-3α/β (Ser21/9) Antibody
- 9336 Phospho-GSK-3β (Ser9) Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9315 GSK-3β (27C10) Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
