Cell Signaling Technology

Product Pathways - MAPK Signaling

RSK2 Antibody #9340

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 90 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

RSK2 Antibody detects endogenous levels of total RSK2 protein. It does not cross-react with the RSK1 or RSK3 isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Glu719 of human RSK2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of purified RSK1, RSK2 and RSK3 proteins using RSK1 Antibody #9333 (upper), RSK2 Antibody (middle) or RSK3 Antibody #9343 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293, L-929 and PC12 cells using RSK2 Antibody.

Background

The 90 kDa ribosomal S6 kinases (RSK1?4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a C-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363 and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones and neurotransmitters (3).

Stimulation by various growth factors leads to activation of RSK2, which is a critical downstream effector kinase in several pathways. EGF stimulation leads to phosphorylation of CREB at Ser133 and phosphorylation of histone H3 in vivo by RSK2 (4,5). RSK2 phosphorylation of p53 may help regulate chromatin structure and cell cycle (6). RSK2 is prominently expressed in the brain and is essential for cognitive function and learning. During development, RSK2 regulates the differentiation of osteoblasts and skeletal muscle cells (7,8). Mutations in the corresponding gene are associated with Coffin-Lowry syndrome (CLS), an X-linked disorder characterized by mental retardation and the presence of characteristic facial anomalies (9).

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
  4. De Cesare, D. et al. (1998) Proc Natl Acad Sci USA 95, 12202-7.
  5. Sassone-Corsi, P. et al. (1999) Science 285, 886-91.
  6. Cho, Y.Y. et al. (2005) Cancer Res 65, 3596-603.
  7. Yang, X. et al. (2004) Cell 117, 387-98.
  8. Cho, Y.Y. et al. (2007) J Biol Chem 282, 8380-92.
  9. Delaunoy, J.P. et al. (2006) Clin Genet 70, 161-6.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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