Product Pathways - MAPK Signaling
Phospho-p90RSK (Ser380) Antibody #9341
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IP | H M R Mk Hm | 90 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Hm=Hamster
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-p90RSK (Ser380) Antibody detects endogenous levels of p90RSK only when phosphorylated at Ser380. This antibody does not recognize p90RSK phosphorylated at other sites. It shows some cross-reactivity with RSK2 and RSK3 when phosphorylated at the homologous serine residues.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser380 of human p90RSK. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 cells, untreated or TPA-treated (200 nM) for the indicated times, using Phospho-p90RSK (Ser380) Antibody (upper) or control RSK1/RSK2/RSK3 (lower).
Background
The 90 kDa ribosomal S6 kinases (RSK1?4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a C-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363 and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones and neurotransmitters (3).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
Application References
- Delehedde, M. et al. (2000) Fibroblast growth factor-2 stimulation of p42/44MAPK phosphorylation and IkappaB degradation is regulated by heparan Sulfate/Heparin in rat mammary fibroblasts. J. Biol. Chem. 275, 33905-33910. This article references the use of Phospho-p90RSK (Ser380) Antibody in the following applications: Western Blotting
- Majka, M. et al. (2000) Stromal-derived factor 1 and thrombopoietin regulate distinct aspects of human megakaryopoiesis. Blood 96, 4142-4151. This article references the use of Phospho-p90RSK (Ser380) Antibody in the following applications: Western Blotting
- Tomas-Zuber, M. et al. (2000) Control sites of ribosomal S6 kinase B and persistent activation through tumor necrosis factor. J. Biol. Chem. 275, 23549-23558. This article references the use of Phospho-p90RSK (Ser380) Antibody in the following applications: Western Blotting
- Hans, A. (2001) Borna disease virus persistent infection activates mitogen-activated protein kinase and blocks neuronal differentiation of pc12 cells. J. Biol. Chem. 276, 7258-7265. This article references the use of Phospho-p90RSK (Ser380) Antibody in the following applications: Western Blotting
- Zaru, R. et al. (2007) Nat Immunol 8, 1227-35. This article references the use of Phospho-p90RSK (Ser380) Antibody in the following applications: Western Blotting
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Companion Products
- 9344 Phospho-p90RSK (Thr359/Ser363) Antibody
- 9348 Phospho-RSK3 (Thr356/Ser360) Antibody
- 9346 Phospho-p90RSK (Thr573) Antibody
- 2211 Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
- 2215 Phospho-S6 Ribosomal Protein (Ser240/244) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9347 RSK1/RSK2/RSK3 Antibody
- 9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb
