Product Pathways - MAPK Signaling
Phospho-p90RSK (Thr359/Ser363) Antibody #9344
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 90 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-p90RSK (Thr359/Ser363) Antibody detects endogenous levels of p90RSK only when phosphorylated at Thr359/Ser363. This antibody does not recognize p90RSK phosphorylated at other sites. It shows some cross-reactivity with RSK3 when phosphorylated at the homologous sites, but no cross-reactivity with phospho-RSK2 or -MSK1.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr359/Ser363 of rat RSK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa and C6 cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr359/Ser363) Antibody (upper) or control RSK1/RSK2/RSK3 Rabbit mAb #9355 (lower).
Background
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
Application References
- Prieto, A.L. et al. (2007) Neuroscience 150, 319-34. Applications: Western Blotting
- Small, G. W. et al. (2003) Repression of Mitogen-Activated Protein Kinase (MAPK) Phosphatase-1 by Anthracyclines Contributes to their Antiapoptotic Activation of p44/42-MAPK. J Pharmacol Exp Ther 307 (3), 861-869. Applications: Western Blotting
- Wu, J. et al. (2002) Attenuation of protein kinase C and cAMP-dependent protein kinase signal transduction in the neurogranin knockout mouse. J. Biol. Chem. 277, 19498-19505. Applications: Western Blotting
- Zhu, Y. et al. (2002) Transforming growth factor-beta 1 increases bad phosphorylation and protects neurons against damage. J. Neurosci. 22, 3898-3909. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9326 p90RSK Antibody Sampler Kit
- 3556 Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb
- 9335 Phospho-p90RSK (Ser380) (9D9) Rabbit mAb
- 9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb
- 9333 RSK1 Antibody
- 9340 RSK2 Antibody
- 9343 RSK3 Antibody
- 9341 Phospho-p90RSK (Ser380) Antibody
- 9346 Phospho-p90RSK (Thr573) Antibody
- 9347 RSK1/RSK2/RSK3 Antibody
- 9348 Phospho-RSK3 (Thr356/Ser360) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
