Product Pathways - MAPK Signaling

Phospho-p90RSK (Thr359/Ser363) Antibody #9344

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Applications	Reactivity	MW (kDa)	Source
W IP IHC-P	H M R Mk	90	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-p90RSK (Thr359/Ser363) Antibody detects endogenous levels of p90RSK only when phosphorylated at Thr359/Ser363. This antibody does not recognize p90RSK phosphorylated at other sites. It shows some cross-reactivity with RSK3 when phosphorylated at the homologous sites, but no cross-reactivity with phospho-RSK2 or -MSK1.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr359/Ser363 of rat RSK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The 90 kDa ribosomal S6 kinases (RSK1?4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a C-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363 and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones and neurotransmitters (3).

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

Application References

• Wu, J. et al. (2002) Attenuation of protein kinase C and cAMP-dependent protein kinase signal transduction in the neurogranin knockout mouse. J. Biol. Chem. 277, 19498-19505. This article references the use of Phospho-p90RSK (Thr359/Ser363) Antibody in the following applications: Western Blotting
• Small, G. W. et al. (2003) Repression of Mitogen-Activated Protein Kinase (MAPK) Phosphatase-1 by Anthracyclines Contributes to their Antiapoptotic Activation of p44/42-MAPK. J Pharmacol Exp Ther 307 (3), 861-869. This article references the use of Phospho-p90RSK (Thr359/Ser363) Antibody in the following applications: Western Blotting
• Zhu, Y. et al. (2002) Transforming growth factor-beta 1 increases bad phosphorylation and protects neurons against damage. J. Neurosci. 22, 3898-3909. This article references the use of Phospho-p90RSK (Thr359/Ser363) Antibody in the following applications: Western Blotting
• Prieto, A.L. et al. (2007) Neuroscience 150, 319-34. This article references the use of Phospho-p90RSK (Thr359/Ser363) Antibody in the following applications: Western Blotting

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