Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-p90RSK (Thr573) Antibody #9346

Applications Reactivity MW (kDa) Source
W IF-IC H M R Hm X (C) (Z) 90 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  C=Chicken  Hm=Hamster  X=Xenopus  Z=Zebra Fish
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-p90RSK (Thr573) Antibody detects endogenous p90RSK protein only when phosphorylated at Thr573.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr573 of human p90RSK. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, PC12 and NIH/3T3 cells, untreated or TPA-treated, using Phospho-p90RSK (Thr573) Antibody.

IHC-FL (floating)

IHC-FL (floating)

Confocal images of immunohistochemical staining with Phospho-p90RSK (Thr573) Antibody (green) and propidium iodide (red) in rat dentate gyrus regions, using sections from control (left) and 15 minute transient cerebral ischemia followed by 30 minutes of reperfusion (right). (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida.)

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TPA-treated (right), using Phospho-p90RSK (Thr573) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).


Background

The 90 kDa ribosomal S6 kinases (RSK1?4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a C-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363 and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones and neurotransmitters (3).

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

Application References

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