Product Pathways - MAPK Signaling
Phospho-p90RSK (Thr573) Antibody #9346
PhosphoSitePlus® protein, site, and accession data: p90RSK
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R (Hm) (C) (X) (Z) | Endogenous | 90 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
C=Chicken
X=Xenopus
Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-p90RSK (Thr573) Antibody detects endogenous p90RSK protein only when phosphorylated at Thr573.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr573 of human p90RSK. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr573) Antibody (upper) or RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (lower).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TPA-treated (right), using Phospho-p90RSK (Thr573) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Background
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
Application References
- Kosako, H. et al. (2009) Nat Struct Mol Biol 16, 1026-35. Applications: Western Blotting
- Han, M.Y. et al. (2007) Mol Cell Biol 27, 8190-204. Applications: Western Blotting
- Zaru, R. et al. (2007) Nat Immunol 8, 1227-35. Applications: Western Blotting
- Zhang, Y. et al. (2002) RANTES-mediated chemokine transcription in astrocytes involves activation and translocation of p90 ribosomal S6 protein kinase (RSK). J. Biol. Chem. 277, 19042-19048. Applications: Western Blotting
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Companion Products
- 9326 p90RSK Antibody Sampler Kit
- 3556 Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb
- 9335 Phospho-p90RSK (Ser380) (9D9) Rabbit mAb
- 9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb
- 9333 RSK1 Antibody
- 9340 RSK2 Antibody
- 9343 RSK3 Antibody
- 9341 Phospho-p90RSK (Ser380) Antibody
- 9344 Phospho-p90RSK (Thr359/Ser363) Antibody
- 9347 RSK1/RSK2/RSK3 Antibody
- 9348 Phospho-RSK3 (Thr356/Ser360) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
