Product Pathways - MAPK Signaling
Phospho-RSK3 (Thr356/Ser360) Antibody #9348
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 90 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-RSK3 (Thr356/Ser360) Antibody detects endogenous levels of RSK3 only when phosphorylated at threonine 356 and serine 360. The antibody does not recognize RSK3 phosphorylated at other sites. It shows some cross-reactivity with RSK1 and RSK2 when phosphorylated at the homologous sites.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr356/Ser360 of human RSK3. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TPA-treated, using Phospho-RSK3 (Thr356/Ser360) Antibody.
Background
The 90 kDa ribosomal S6 kinases (RSK1?4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a C-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363 and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones and neurotransmitters (3).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
Application References
- Zaru, R. et al. (2007) Nat Immunol 8, 1227-35. This article references the use of Phospho-RSK3 (Thr356/Ser360) Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9341 Phospho-p90RSK (Ser380) Antibody
- 9344 Phospho-p90RSK (Thr359/Ser363) Antibody
- 9346 Phospho-p90RSK (Thr573) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9347 RSK1/RSK2/RSK3 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb
- 9335 Phospho-p90RSK (Ser380) (9D9) Rabbit mAb
- 9324 Phospho-RSK2 (Tyr529) Antibody
- 9333 RSK1 Antibody
- 9340 RSK2 Antibody
- 9343 RSK3 Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
