Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

PhosphoPlus® Stat5 (Tyr694) Antibody Kit #9350

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
Stat5 Antibody #9363 100 µl W IP ChIP H M R Mk 90 Rabbit
Phospho-Stat5 (Tyr694) Antibody #9351 100 µl W F ChIP H M (R) (B) 90 Rabbit
Stat5 Control Cell Extracts #9353 100 µl Human
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat
Anti-biotin, HRP-linked Antibody #7075 100 µl Goat
Biotinylated Protein Ladder Detection Pack #7727 100 µl
20X LumiGLO® Reagent and 20X Peroxide #7003 5 ml each

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry  ChIP=Chromatin IP
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Phospho-Stat5 (Tyr694) Antibody detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699. This antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins, but does cross-react with EGFR. Stat5 Antibody detects endogenous levels of total Stat5a and Stat5b.

Description

The PhosphoPlus® Stat5 (Tyr694) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of Stat5a at Tyr694 and Stat5b at Tyr699.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr694 of mouse Stat5a (Phospho-Stat5 (Tyr694) Antibody) or a synthetic peptide corresponding to an amino-terminal region of Stat5. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

  1. Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369.
  2. Wakao, H. et al. (1994) EMBO J. 13, 2182-2191.
  3. Okutani, Y. et al. (2001) Oncogene 20, 6643-6650.
  4. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861.
  5. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
  6. Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.

Application References

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Protocols

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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