Product Pathways - MAPK Signaling
RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355
|W IP IF-IC||H M R Mk GP||Endogenous||90||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
RSK1/RSK2/RSK3 (32D7) Rabbit mAb detects endogenous levels of total RSK1/RSK2/RSK3 proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp659 of human p90RSK.
Western blot analysis of extracts from HeLa, L929 and AR42J cells, using RSK1/RSK2/RSK3 (32D7) Rabbit mAb.
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.