Product Pathways - Jak/Stat Pathway
Phospho-Stat6 (Tyr641) Antibody #9361
PhosphoSitePlus® protein, site, and accession data: STAT6
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC F | H (B) | Endogenous | 110 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
* Product-specific protocol.
Specificity / Sensitivity
Phospho-Stat6 (Tyr641) Antibody detects endogenous levels of Stat6 only when phosphorylated at tyrosine 641. The antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr641 of human Stat6. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Daudi cells, treated with IL-4 (100 ng/ml) for the indicated times using Phospho-Stat6 (Tyr641) Antibody (upper) or Stat6 antibody (lower).
Flow Cytometry
Flow cytometric analysis of Ramos cells, untreated (blue) or IL-4 treated (green), using Phospho-Stat6 (Tyr641) Antibody compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of ACHN cells serum-starved (left) or IL-4-treated (right) using Phospho-Stat6(Tyr641) Antibody (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Background
Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).
- Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
- Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
- Hou, J. et al. (1994) Science 265, 1701-1706.
- Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
- Takeda, K. et al. (1996) Nature 380, 627-630.
- Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
- Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.
Application References
- Bartoli, M. et al. (2000) Vascular endothelial growth factor activates STAT proteins in aortic endothelial cells. J. Biol. Chem. 275, 33189-33192. Applications: Western Blotting
- Majka, M. et al. (2000) Stromal-derived factor 1 and thrombopoietin regulate distinct aspects of human megakaryopoiesis [In Process Citation]. Blood 96, 4142-4151. Applications: Western Blotting
- Lee, J. et al. (2011) Oncogene , . Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9362 Stat6 Antibody
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
