Product Pathways - Jak/Stat Pathway
Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb #9364
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W | H (Mk) | Endogenous | 100 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb detects endogenous levels of Stat6 only when phosphorylated at Tyr641. This antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins. Cross-reactivity has been observed with receptor tyrosine kinases including phosphorylated PDGF receptor that can lead to inapproriate membrane staining.
Source / Purification
Rabbit monoclonal antibodies were prepared from spleens of rabbits immunized with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Tyr641 of human Stat6.
Western Blotting
Western blot analysis of extracts from HeLa and ACHN cells, untreated or treated with IL-4 (15 min), using Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb (upper) or Stat6 Antibody #9362 (lower).
Background
Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).
- Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
- Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
- Hou, J. et al. (1994) Science 265, 1701-1706.
- Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
- Takeda, K. et al. (1996) Nature 380, 627-630.
- Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
- Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.
Application References
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Companion Products
- 9361 Phospho-Stat6 (Tyr641) Antibody
- 9362 Stat6 Antibody
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- 9145 Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb
- 9359 Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
