Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Cytoskeletal Signaling

Rab7 (D95F2) XP® Rabbit mAb #9367

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 23 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rab7 (D95F2) XP® Rabbit mAb detects endogenous levels of total Rab7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu188 of human Rab7 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rab7 (D95F2) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-MEL-28 cells using Rab7 (D95F2) XP® Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes, but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10).

  1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
  2. Feng, Y. et al. (1995) J Cell Biol 131, 1435-52.
  3. Méresse, S. et al. (1995) J Cell Sci 108 ( Pt 11), 3349-58.
  4. Ceresa, B.P. and Bahr, S.J. (2006) J Biol Chem 281, 1099-106.
  5. Jäger, S. et al. (2004) J Cell Sci 117, 4837-48.
  6. Méresse, S. et al. (1999) EMBO J 18, 4394-403.
  7. Díaz, E. et al. (1997) J Cell Biol 138, 283-90.
  8. Barbero, P. et al. (2002) J Cell Biol 156, 511-8.
  9. Lombardi, D. et al. (1993) EMBO J 12, 677-82.
  10. Riederer, M.A. et al. (1994) J Cell Biol 125, 573-82.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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