Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

GSK-3 Antibody Sampler Kit #9369

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
GSK-3α Antibody # 9338 40 microliters W H M R Mk 51 Rabbit
Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb # 9323 40 microliters W IHC-P IF-IC H M R Mk 46 Rabbit IgG
Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) # 9327 40 microliters W IP H M R Mk 51alpha; 46beta Rabbit IgG
GSK-3β (27C10) Rabbit mAb # 9315 40 microliters W IP IHC-P H M R Mk 46 Rabbit IgG
Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb # 9316 40 microliters W IHC-P H M R Mk 51 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody # 7074 100 microliters Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey

Specificity / Sensitivity

Each antibody in the GSK-3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GSK-3β (27C10) Rabbit mAb #9315.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 (upper) or GSK-3α Antibody #9338 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from wild type (lanes 1,2), GSK-3α (-/-) (lanes 3,4) or GSK-3β (-/-) (lanes 5,6) mouse embryonic fibroblasts (MEFs), untreated or PDGF treated, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb #9323 (upper) and GSK-3α/β Antibody (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).


Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) #9327 (upper) or GSK-3α Antibody #9338 (lower).

Western Blotting

Western Blotting

Western blot analysis of recombinant GST-GSK-3β protein (76kD), or extracts from HeLa and PC3 cells, using GSK-3α Antibody #9338 (left) and GSK-3β Antibody (right).

Description

The GSK-3 Antibody Sampler Kit contains primary and secondary antibodies to perform four Western blots with each antibody.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GSK-3α. Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser9 of human GSK-3β, residues around Ser21 of human GSK-3α, the N terminal sequence of human GSK-3β and to residues around Ser21 of human GSK-3α. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

  1. Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
  3. Cross, D.A. et al. (1995) Nature 378, 785-789.
  4. Nusse, R. (1997) Cell 89, 321-323.
  5. Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.

Application References

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Protocols

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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