Product Pathways - PI3K / Akt Signaling
GSK-3 Antibody Sampler Kit #9369
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|GSK-3α (D80E6) Rabbit mAb #4337||40 µl||W IP||H M R Hm Mk||51||Rabbit IgG|
|Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) #9327||40 µl||W IP||H M R Mk||51, 46||Rabbit IgG|
|GSK-3β (27C10) Rabbit mAb #9315||40 µl||W IP IHC-P||H M R Mk||46||Rabbit IgG|
|Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316||40 µl||W IHC-P||H M R Mk||51||Rabbit IgG|
|Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558||40 µl||W IP IF-IC F||H M R Hm||46||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the GSK3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Phospho-GSK-3ß (Ser9) (D85E12) XP® Rabbit mAb reacts with denatured components of bovine serum, including BSA.
Western blot analysis of extracts from various cell types using GSK-3α (D80E6) Rabbit mAb #4337.
Western blot analysis of extracts from GSK-3α (-/-) (lanes 1,2) and GSK-3β (-/-) (lanes 3,4) mouse embryonic fibroblast (MEF) cells, λ phosphatase or PDGF-treated, using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558 (upper) and α/β-Tubulin Antibody #2148 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).
Western blot analysis of extracts from various cell lines using GSK-3β (27C10) Rabbit mAb #9315.
Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 (upper) or GSK-3α Antibody #9338 (lower).
The GSK-3 Antibody Sampler Kit contains primary and secondary antibodies to perform four Western blots with each antibody.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Ser9 of human GSK-3ß, residues around Ser21 of human GSK-3α, the N terminal sequence of human GSK-3ß or to the sequence of human GSK-3α protein.
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
- Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
- Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
- Cross, D.A. et al. (1995) Nature 378, 785-789.
- Nusse, R. (1997) Cell 89, 321-323.
- Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.
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- 5676 GSK-3α/β (D75D3) XP® Rabbit mAb
- 9832 GSK-3β (3D10) Mouse mAb
For Research Use Only. Not For Use In Diagnostic Procedures.