Product Pathways - PI3K / Akt Signaling
GSK-3 Antibody Sampler Kit #9369
|9369S||1 Kit (5 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|GSK-3α (D80E6) Rabbit mAb #4337||40 µl||W, IP||H, M, R, Hm, Mk||51||Rabbit IgG|
|Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) #9327||40 µl||W, IP||H, M, R, Mk||51, 46||Rabbit IgG|
|GSK-3β (27C10) Rabbit mAb #9315||40 µl||W, IP, IHC-P||H, M, R, Mk||46||Rabbit IgG|
|Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316||40 µl||W, IHC-P||H, M, R, Mk||51||Rabbit|
|Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558||40 µl||W, IP, IF-IC, F||H, M, R, Hm||46||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey
Western blot analysis of extracts from various cell types using GSK-3α (D80E6) Rabbit mAb #4337.
Western blot analysis of extracts from GSK-3α (-/-) (lanes 1,2) and GSK-3β (-/-) (lanes 3,4) mouse embryonic fibroblast (MEF) cells, λ phosphatase or PDGF-treated, using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558 (upper) and α/β-Tubulin Antibody #2148 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).
Western blot analysis of extracts from various cell lines using GSK-3β (27C10) Rabbit mAb #9315.
Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 (upper) or GSK-3α Antibody #9338 (lower).
The GSK-3 Antibody Sampler Kit contains primary and secondary antibodies to perform four Western blots with each antibody.
Specificity / Sensitivity
Each antibody in the GSK3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Phospho-GSK-3ß (Ser9) (D85E12) XP® Rabbit mAb reacts with denatured components of bovine serum, including BSA.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Ser9 of human GSK-3ß, residues around Ser21 of human GSK-3α, the N terminal sequence of human GSK-3ß or to the sequence of human GSK-3α protein.
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.