Product Pathways - Ca / cAMP / Lipid Signaling
Phospho-PKC (pan) (βII Ser660) Antibody #9371
|W||H M R Mk||Endogenous||78, 80, 82, 85||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-PKC (pan) (βII Ser660) Antibody detects endogenous levels of PKC α, β I, β II, δ, ε, η and θ isoforms only when phosphorylated at a carboxy-terminal residue homologous to serine 660 of PKC β II. This antibody does not detect PKC phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser660 of human PKC β II. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of Baculovirus expressed PKCβ and PKCβ Ser660/Ala mutant, using Phospho-PKC (pan) (βII Ser660) Antibody (upper) or control PKCβ antibody (lower).
Western blot analysis of extracts from TPA, Go6983 and/or Bisindolylmaleimide treated 293 cells, using Phospho-PKC (pan) (βII Ser660) Antibody.
Western blot analysis of Baculovirus expressed PKC isoforms, using Phospho-PKC (pan) (βII Ser660) Antibody.
Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7).
- Nishizuka, Y. (1984) Nature 308, 693-698.
- Keranen, L.M. et al. (1995) Curr. Biol. 5, 1394-1403.
- Mellor, H. and Parker, P.J. (1998) Biochem J. 332 (Pt 2), 281-292.
- Ron, D. and Kazanietz, M.G. (1999) FASEB J. 13, 1658-1676.
- Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Rep. 1, 399-403.
- Baron, C.L. and Malhotra, V. (2002) Science 295, 325-328.
- Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-11070.
- Deszo, E. L. et al. (2001) Cd45 negatively regulates monocytic cell differentiation by inhibiting phorbol 12-myristate13-acetate-dependent activation and tyrosine phosphorylation of protein kinase c delta. J. Biol. Chem. 276, 10212-10217. Applications: Western Blotting
- Sweeney, C. et al. (2001) Growth Factor-specific Signaling Pathway Stimulation and Gene Expression Mediated by ErbB Receptors. J. Biol. Chem. 276, 22685-22698. Applications: Western Blotting
- Iwabu, A. et al. (2004) Epidermal growth factor induces fibroblast contractility and motility via a protein kinase C delta-dependent pathway. J. Biol. Chem. 279, 14551-14560. Applications: Western Blotting
- Godbout, J. P. et al. (2002) Methylglyoxal Enhances Cisplatin-induced Cytotoxicity by Activating Protein Kinase C-delta. J. Biol. Chem 277 (4), 2554-2561. Applications: Western Blotting
- Leinweber, B. et al. (2000) Regulation of protein kinase C by the cytoskeletal protein calponin. J. Biol. Chem. 275, 40329-40336. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2056 PKCα Antibody
- 2058 PKCδ Antibody
- 2261 Phospho-(Ser) PKC Substrate Antibody
- 9372 PKCζ Antibody
- 9374 Phospho-PKCδ (Thr505) Antibody
- 9375 Phospho-PKCα/β II (Thr638/641) Antibody
- 9376 Phospho-PKCδ/θ (Ser643/676) Antibody
- 9377 Phospho-PKCθ (Thr538) Antibody
- 9378 Phospho-PKCζ/λ (Thr410/403) Antibody
- 9379 Phospho-PKC (pan) (gamma Thr514) Antibody
- 9921 Phospho-PKC Antibody Sampler Kit
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.