Product Pathways - Antibody Sampler Kits
Cytokeratin Antibody Sampler Kit #9384
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Keratin 7 Antibody #3473 | 40 µl | W | H (Mk) | 52 | Rabbit |
| Keratin 17 (D73C7) XP® Rabbit mAb #4543 | 40 µl | W IF-IC F | H M R Mk (Dg) | 48 | Rabbit IgG |
| Keratin 8/18 (C51) Mouse mAb #4546 | 40 µl | W IHC-P F | H Mk | 46 Keratin 18. 55 Keratin 8. | Mouse IgG1 |
| Keratin 18 (DC10) Mouse mAb #4548 | 40 µl | W IHC-P IF-IC F | H | 46 | Mouse IgG1 |
| Keratin 19 (BA17) Mouse mAb #4558 | 40 µl | W IHC-P | H | 40 | Mouse IgG1 |
| Pan-Keratin (C11) Mouse mAb #4545 | 40 µl | W IHC-P IF-IC IF-P F | H R Mk | 46-62 | Mouse IgG1 |
| Anti-mouse IgG, HRP-linked Antibody #7076 | 100 µl | Horse | |||
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
IF-P=Immunofluorescence (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
The pan-keratin (C11) mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins. Each of the remaining antibodies included in this kit detect endogenous levels of the specified keratin protein and do not cross-react with other keratin proteins.
Western Blotting
Western blot analyses of extracts of various cell lines using Keratin 7 Antibody #3473. As expected, keratin 7 is absent in MCF-7 cells.
Western Blotting
Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) XP® Rabbit mAb #4543. As expected, keratin 17 is absent in A549 cells.
Western Blotting
Western blot analysis of extracts from various cell lines using Pan-Keratin (C11) Mouse mAb #4545.
Western Blotting
Western blot analysis of extracts from various cell lines using Keratin 8/18 (C51) Mouse mAb #4546.
Western Blotting
Western blot analysis of extracts from HeLa and A-431 cells using Keratin 18 (DC10) Mouse mAb #4548.
Western Blotting
Western blot analysis of extracts from various cell types using Keratin 19 (BA17) Mouse mAb #4558. As expected, the protein is absent in A-431 and HeLa cells.
Description
The Cytokeratin Antibody Sampler Kit provides an economical means to evaluate the presence and status of selected keratin proteins. The kit provides enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
Source / Purification
Pan-Keratin Mouse mAb (C11) is produced by immunizing animals with a cytoskeleton preparation from A431 cells. Keratin 8/18 (C51) Mouse mAb is produced by immunizing animals with a cytoskeleton preparation from HeLa cells. Keratin 18 (DC10) Mouse mAb is produced by immunizing animals with human PMC-42 breast carcinoma cells. Keratin 19 (BA17) Mouse mAb is produced by immunizing animals with detergent-insoluble extract of human mammary epithelial organoids. Keratin 7 Antibody is generated by immunizing animals with a synthetic peptide corresponding to amino acids at the amino terminus of human keratin 7. Keratin 17 (D73C7) XP® Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human keratin 17. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 3473:
- Western Blotting
- 4543:
- Flow, Immunofluorescence, Western Blotting
- 4545:
- Flow, IHC / Paraffin, Immunofluorescence, Western Blotting
- 4546:
- Flow, IHC / Paraffin, Western Blotting
- 4548:
- Flow, IHC / Paraffin, Immunofluorescence, Western Blotting
- 4558:
- IHC / Paraffin, Western Blotting
Companion Products
- 3479 Keratin 19 Antibody
- 3984 Keratin 17/19 (D32D9) XP® Rabbit mAb
- 3519 Phospho-Keratin 17 (Ser44) Antibody
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7075 Anti-biotin, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 7722 Blue Loading Buffer Pack
- 7723 Red Loading Buffer Pack
- 7074 Anti-rabbit IgG, HRP-linked Antibody
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
