Product Pathways - Motif Antibodies

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Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) #9391

Applications	Reactivity	Sensitivity	Isotype
W IHC-P E-P	All	Endogenous	Mouse IgM

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

9391:

ELISA Peptide, IHC / Paraffin, Western Blotting

Specificity / Sensitivity

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) detects phospho-threonine only when followed by proline. It reacts with proteins and peptides phosphorylated at the Thr-Pro motif in an otherwise highly context-independent fashion. The antibody is phospho-specific, but does not recognize phospho-threonine in the absence of an adjacent proline. The antibody does not react with phospho-tyrosine but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-threonine-proline-containing peptides. This antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.

Background

The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To study and discover new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline.

As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101) recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by western analysis of nocodazole-treated Jurkat cell extracts resolved on 2-D gels.

1. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
2. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
3. Nurse, P. (2000) Cell 100, 71-8.
4. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
5. Yang, C.C. et al. (1998) J Protein Chem 17, 329-35.
6. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.

Application References

• Xu, P. and Derynck, R. (2010) Mol Cell 37, 551-66. Applications: Western Blotting
• Mishima, M. et al. (2004) Nature 430, 908-13. Applications: Western Blotting
• Sugiura, R. et al. (2003) Nature 424, 961-5. Applications: Western Blotting
• Monje, P. et al. (2003) Mol Cell Biol 23, 7030-43. Applications: Western Blotting
• Thiel, D.A. et al. (2002) Curr Biol 12, 1227-32. Applications: Western Blotting
• Matter, N. et al. (2002) Nature 420, 691-5. Applications: Western Blotting
• Díaz-Rodríguez, E. et al. (2002) Mol Biol Cell 13, 2031-44. Applications: Western Blotting
• Marinissen, M.J. et al. (2001) Genes Dev 15, 535-53. Applications: Western Blot
• Wada, T. et al. (2001) J Biol Chem 276, 30892-7. Applications: Western Blotting
• Hall, C. et al. (2001) Mol Cell Biol 21, 1854-65. Applications: Western Blot
• Rashid, T. et al. (2001) J Biol Chem 276, 49043-52. Applications: Western Blot
• Conover, C.A. et al. (2000) Endocrinology 141, 3098-103. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9381 Phospho-Threonine Antibody (P-Thr-Polyclonal)
• 2261 Phospho-(Ser) PKC Substrate Antibody
• 9601 Phospho-(Ser) 14-3-3 Binding Motif Antibody
• 9614 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb
• 9624 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb
• 2351 Phospho-Threonine-X-Arginine Antibody
• 9611 Phospho-(Ser/Thr) Akt Substrate Antibody
• 9621 Phospho-(Ser/Thr) PKA Substrate Antibody
• 9631 Phospho-(Ser/Thr) Phe Antibody
• 7072 Phototope^®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
• 7076 Anti-mouse IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

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For Research Use Only. Not For Use In Diagnostic Procedures.