Product Pathways - Motif Antibodies
Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) #9391
|9391S||100 µl (50 western blots)||---||In Stock||---|
|9391||carrier free and custom formulation / quantity||email request|
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|W||1:5000||All Species Expected||Endogenous||Mouse IgM|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), E-P=ELISA (Peptide)
Specificity / Sensitivity
Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) detects phospho-threonine only when followed by proline. It reacts with proteins and peptides phosphorylated at the Thr-Pro motif in an otherwise highly context-independent fashion. The antibody is phospho-specific, but does not recognize phospho-threonine in the absence of an adjacent proline. The antibody does not react with phospho-tyrosine but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic phospho-threonine-proline-containing peptides. This antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.
Western blot analysis of extracts from Jurkat cells, untreated or nocadazole-treated (1 µg/ml for 12 hours prior to lysis), using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101). Proteins were separated by 2D electrophoresis prior to blotting.
Western blot analysis of extracts from COS cells, untreated or serum and okadaic acid-treated, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) (left). Right panel shows total protein staining.
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-Threonine-Prolin Mouse mAb (P-Thr-Pro-101).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phospho-threonine-proline motifs, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101).
The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To study and discover new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline.
As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101) recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by western analysis of nocodazole-treated Jurkat cell extracts resolved on 2-D gels.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
- Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
- Nurse, P. (2000) Cell 100, 71-8.
- Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
- Yang, C.C. et al. (1998) J Protein Chem 17, 329-35.
- Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
- Xu, P. and Derynck, R. (2010) Mol Cell 37, 551-66. Applications: Western Blotting.
- Mishima, M. et al. (2004) Nature 430, 908-13. Applications: Western Blotting.
- Sugiura, R. et al. (2003) Nature 424, 961-5. Applications: Western Blotting.
- Monje, P. et al. (2003) Mol Cell Biol 23, 7030-43. Applications: Western Blotting.
- Thiel, D.A. et al. (2002) Curr Biol 12, 1227-32. Applications: Western Blotting.
- Matter, N. et al. (2002) Nature 420, 691-5. Applications: Western Blotting.
- Díaz-Rodríguez, E. et al. (2002) Mol Biol Cell 13, 2031-44. Applications: Western Blotting.
- Wada, T. et al. (2001) J Biol Chem 276, 30892-7. Applications: Western Blotting.
- Conover, C.A. et al. (2000) Endocrinology 141, 3098-103. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at firstname.lastname@example.org.