Cell Signaling Technology

Product Pathways - Neuroscience

CRMP-2 Antibody #9393

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Endogenous 55-65 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CRMP-2 Antibody detects endogenous levels of total CRMP-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide of human CRMP-2. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using CRMP-2 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of PC-12 cells using CRMP-2 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 and is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5).

  1. Gu, Y. and Ihara, Y. (2000) J Biol Chem 275, 17917-20.
  2. Yoshimura, T. et al. (2005) Cell 120, 137-49.
  3. Fukata, Y. et al. (2002) Nat Cell Biol 4, 583-91.
  4. Goshima, Y. et al. (1995) Nature 376, 509-14.
  5. Cole, A.R. et al. (2004) J Biol Chem 279, 50176-80.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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