Product Pathways - Neuroscience
Phospho-CRMP-2 (Thr514) Antibody #9397
|W||H M R||Endogenous||55-65||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-CRMP-2 (Thr514) Antibody detects endogenous levels of CRMP-2 only when phosphorylated at Thr514.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr514 of human CRMP-2. Antibodies are purified by peptide affinity chromatography.
Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 and is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5).
- Gu, Y. and Ihara, Y. (2000) J Biol Chem 275, 17917-20.
- Yoshimura, T. et al. (2005) Cell 120, 137-49.
- Fukata, Y. et al. (2002) Nat Cell Biol 4, 583-91.
- Goshima, Y. et al. (1995) Nature 376, 509-14.
- Cole, A.R. et al. (2004) J Biol Chem 279, 50176-80.
- Crews, L. et al. (2011) Mol Neurodegener 6, 67. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.