Cell Signaling Technology

Product Pathways - Translational Control

DDX6/RCK Antibody #9407

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC F H M R Mk Endogenous 54 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

DDX6/RCK Antibody detects endogenous levels of total DDX6/RCK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DDX6/RCK protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using DDX6/RCK Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using DDX6/RCK Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of UV-treated C2C12 cells using DDX6/RCK Antibody (green) and α-Tubulin (DM1A) Mouse mAb #3873 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

DDX6, also known as RCK and p54, was identified as a proto-oncogene product and is a member of the ATP-dependent DEAD box helicase family (1,2). This protein interacts with translation initiation factor eIF4E in the cytoplasmic P-bodies (3) and represses mRNA translation (4). DDX6 is a component of the miRNA induced silencing complex (miRISC) and interacts with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) proteins in vitro and in vivo (5), functioning in miRNA-mediated translational repression (5). Depletion of DDX6 leads to the disruption of cytoplasmic P-bodies indicating that it is required for P-body formation (5).

  1. Akao, Y. et al. (1995) Cancer Res 55, 3444-9.
  2. Weston, A. and Sommerville, J. (2006) Nucleic Acids Res 34, 3082-94.
  3. Andrei, M.A. et al. (2005) RNA 11, 717-27.
  4. Minshall, N. and Standart, N. (2004) Nucleic Acids Res 32, 1325-34.
  5. Chu, C.Y. and Rana, T.M. (2006) PLoS Biol 4, e210.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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