Product Pathways - Motif Antibodies
Phospho-Tyrosine Mouse mAb (P-Tyr-102) #9416
| Applications | Reactivity | Sensitivity | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P F E-P | All | Endogenous | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Specificity / Sensitivity
Phospho-Tyrosine Mouse mAb (P-Tyr-102) is a high affinity IgG1 monoclonal antibody. ELISAs using a wide variety of phospho-peptides show that P-Tyr-102 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence.2D gel Western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-102 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-102's fine specificity in terms of the sequence context in which it can recognize phospho-tyrosine seems to differ slightly from that of P-Tyr-100 #9411. P-Tyr-102 does not recognize peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Monoclonal antibody is produced by immunizing mice with synthetic phospho-Tyr-containing peptides (KLH-coupled).
Western Blotting
Western blot analysis of extracts from sodium vanadate treated (3 mM for 0.5 hour) NIH/3T3 cells, using Phospho-Tyrosine Mouse mAb (P-Tyr-102).
Western Blotting
Western blot analysis of extracts from Jurkat cells treated with 1 mM pervanadate for 30 minutes prior to lysis. Proteins were separated by 2D electrophoresis prior to blotting.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Tyrosine Mouse mAb (P-Tyr-102).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft untreated (left) or lambda-phosphatase-treated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-102).
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or pervanadate-treated (green), using Phospho-Tyrosine Mouse mAb (P-Tyr-102) compared with a nonspecific negative control antibody (red).
ELISA-Peptide
Phospho-Tyrosine Mouse mAb (P-Tyr-102) ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (Y* denotes phosphorylated tyrosine.)
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine Mouse mAbs developed by CST (P-Tyr-100, #9411 and P-Tyr-102, #9416) provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphoyrlation and monitoring tyrosine kinase activity in high throughput drug discovery.
- Schlessinger, J. (2000) Cell 103, 211-25.
- Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
- Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
- Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.
Application References
- Ma, Y.C. et al. (2000) Cell 102, 635-46. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-102) in the following applications: Western Blot
- Weinberg, J.M. et al. (2001) Am J Pathol 158, 2153-64. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-102) in the following applications: Western Blotting
- Nakamura, T. et al. (2002) FEBS Lett 521, 190-4. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-102) in the following applications: Western Blotting
- Weiser, J.N. et al. (2001) Infect Immun 69, 5430-9. This article references the use of Phospho-Tyrosine Mouse mAb (P-Tyr-102) in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9414 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate)
- 9415 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 647 Conjugate)
- 9417 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)
- 9418 Phospho-Tyrosine Mouse mAb (P-Tyr-102) (Fluorescein Conjugate)
- 3821 Phospho-(Tyr) p85 PI3K Binding Motif Antibody
- 9419 Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9381 Phospho-Threonine Antibody (P-Thr-Polyclonal)
- 9691 Nitro-Tyrosine Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
