Product Pathways - Motif Antibodies
Phospho-Tyrosine Mouse mAb (P-Tyr-102) #9416
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IP IHC-P F E-P | All | Endogenous | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
Specificity / Sensitivity
Phospho-Tyrosine Mouse mAb (P-Tyr-102) is a high affinity IgG1 monoclonal antibody. ELISAs using a wide variety of phospho-peptides show that P-Tyr-102 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence.2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-102 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-102's fine specificity in terms of the sequence context in which it can recognize phospho-tyrosine seems to differ slightly from that of P-Tyr-100 #9411. P-Tyr-102 does not recognize peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic phospho-Tyr-containing peptides .
Western Blotting
Western blot analysis of extracts from sodium vanadate treated (3 mM for 0.5 hour) NIH/3T3 cells, using Phospho-Tyrosine Mouse mAb (P-Tyr-102).
Western Blotting
Western blot analysis of extracts from Jurkat cells treated with 1 mM pervanadate for 30 minutes prior to lysis. Proteins were separated by 2D electrophoresis prior to blotting.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Tyrosine Mouse mAb (P-Tyr-102).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft untreated (left) or lambda-phosphatase-treated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-102).
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or pervanadate-treated (green), using Phospho-Tyrosine Mouse mAb (P-Tyr-102) compared with a nonspecific negative control antibody (red).
ELISA-Peptide
Phospho-Tyrosine Mouse mAb (P-Tyr-102) ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (Y* denotes phosphorylated tyrosine.)
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
- Schlessinger, J. (2000) Cell 103, 211-25.
- Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
- Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
- Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.
Application References
- Ma, Y.C. et al. (2000) Cell 102, 635-46. Applications: Western Blot
- Weinberg, J.M. et al. (2001) Am J Pathol 158, 2153-64. Applications: Western Blotting
- Nakamura, T. et al. (2002) FEBS Lett 521, 190-4. Applications: Western Blotting
- Weiser, J.N. et al. (2001) Infect Immun 69, 5430-9. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9414 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate)
- 9415 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 647 Conjugate)
- 9417 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)
- 9419 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate)
- 9381 Phospho-Threonine Antibody (P-Thr-Polyclonal)
- 9691 Nitro-Tyrosine Antibody
- 8102 SignalSlide® Phospho-EGF Receptor IHC Controls
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
