Product Pathways - Motif Antibodies
Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) #9417
| Applications | Reactivity | Source | Isotype |
|---|---|---|---|
| W IP IHC-P | All | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: All=All species expected
Specificity / Sensitivity
Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) is prepared by biotinylation of the antibody via primary amines. Biotinylated antibody is useful for detection or purification of tyrosine-phosphorylated proteins using avidin-biotin binding. This antibody does not cross-react with proteins phosphorylated at serine or threonine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Monoclonal antibody is derived from mice immunized with phospho-Tyr-containing peptides (KLH coupled). Antibody is purified by protein A chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells untreated and treated with sodium vanadate (1 mM for 30 minutes), using 75 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) (A), then detected by Anti-biotin Antibody, HRP-linked #7075. Western blot analysis of the same extract using 750 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411, then detected by Anti-mouse IgG, HRP-linked Antibody #7076 (B).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-Tyrosine mAb (P-Tyr-100) (Biotinylated).
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine Mouse mAbs developed by CST (P-Tyr-100, #9411 and P-Tyr-102, #9416) provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphoyrlation and monitoring tyrosine kinase activity in high throughput drug discovery.
- Schlessinger, J. (2000) Cell 103, 211-225.
- Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-365.
- Ward, S.G. et al. (1992) J. Biol. Chem. 267, 23862-23869.
- Glenney, J.R. et al. (1988) J. Immunol. Methods. 109, 277-285.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9414 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate)
- 9415 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 647 Conjugate)
- 9416 Phospho-Tyrosine Mouse mAb (P-Tyr-102)
- 9418 Phospho-Tyrosine Mouse mAb (P-Tyr-102) (Fluorescein Conjugate)
- 9419 Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 7075 Anti-biotin, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7900 PhosphoScan® Kit (P-Tyr-100)
- 7902 Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads(for use with PhosphoScan® Technology)
License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.
